Studies On All-Female Common Carp Breeding And Sex-Related DNA Marker

The all-female common carp breeding is significant in academic and economic value. The major way of all-female breeding is to combine artificial gynogenesis with sex reversal, in which the key technique is the identification between gynogenesis fish and hybrid individuals. Another way is to treat mixed population of male and female with testosterone firstly, and then identify the pseudo-male by sex-related DNA marker. The precondition is to find sex-related DNA marker. Jian carp, with excellent economic traits, is an extensively cultured variety of common carp in China. As a result, Jian carp was chosen as experimental material in this paper.Artificial gynogenesis of Jian carp (Cyprinus carpio var. jian) induced by red crucian carp (Carassius auratus var. red) sperm inactivated by UV-ray, was carried out. 241 individuals of F₁ progeny were obtained from the gynogenesis, which were classified into I type ( body form with Jian carp ) and II type ( body form with hybrid ) by morphological characteristics. 5 individuals were selected from the two types separately for RAPD analysis. With 10 random primers, 35 bands were obtained in Jian carp, 31 bands in red crucian carp, and 26 bands were shared by Jian carp and red crucian carp. Results showed that 4 individuals of the I type only had the bands of Jian carp and no bands of red crucian carp, which were fish originated from gynogenesis of Jian carp. One individual of the I type had the bands originated mostly from Jian carp except one band from red crucian carp, which was probably a partial hybrid because some DNA fragments of sperms integrated into egg nucleus. The bands both of Jian carp and red crucian carp were found in all individuals of the II type, which were hybrids because of incomplete inactivation of sperms. The study indicated that RAPD technique is useful and practical for the identification between gynogenesis fish and hybrid individuals.RAPD and AFLP technique were used to screen the sex-related DNA marker through analyzing the DNA differences between female and male of Jian carp. 67 random primers were selected in RAPD analysis. Sex differentiations in population level were found inl 8...