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The Genetic Diversity Between Normal And Special Maize Inbreds Studied By SSRs

Posted on:2007-07-06Degree:MasterType:Thesis
Country:ChinaCandidate:C S ZhongFull Text:PDF
GTID:2143360212459988Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Maize inbreds are xenial. For the hybridization along with continuous gene exchange among different plants creating a new hereditary variation pool and thereafter on the effect of natural selection and artificial selection, it creates a new germ plasm which is different from the original germ plasm at quite a large extent. Therefore the morphologic variation range of planted maize inbreds is quite prodigious. According to the characters of endosperm and the presence or absence of the palea/lemma hull, maize inbred lines were classified into 8 groups in 1933, including Zea mays L. indurata Sturt, Zea mays L. indentata Sturt, Zea mays L. amylacea Sturt, Zea mays L. ceratina Kulesh, Zea mays L. saccharata Sturt, Zea mays L. everta Sturt, Zea mays L. amyleo-saccharata Sturt, Zea mays L. tunicata Sturt and so on. Since 1990s, the Maize Research Team in Institute of Biotechnology and Nuclear Technology (Belongs to Sichuan Academy of Agriculture Sciences) has collected and created a sets of maize inbred lines, including Zea mays L. indurata Sturt,Zea mays L. indentata Sturt , Zea mays L. ceratina Kulesh, Zea mays L. saccharata Sturt and Zea mays L. everta Sturt, the genetic relationships of which were studied by using SSR marker.Because of collecting hardly and planting insignicant, other types such as Zea mays L. amylacea Sturt, Zea mays L. amyleo-saccharata Sturt and Zea mays L. tunicata Sturt were not studied in this study.Firstly we studied the genetic relationships among the special maize inbred lines (including Zea mays L. ceratina Kulesh, Zea mays L. saccharata Sturt and Zea mays L. everta Sturt). The genetic relationship of 20 special maize inbred lines was studied by Simple Sequence Repeats (SSRs) marker. Fifteen SSR primers giving stable amplified band patterns produced 67 alleles among the lines tested. The average number of alleles per SSR locus was 4.66 with a range from 2 to 10. The value of polymorphism information content (PIC) for each SSR locus...
Keywords/Search Tags:maize, simple sequence repeats marker, genetic diversity
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