Establishment Of Regeneration System And Genetic Transformation With Floral Genes In Platanus Spp.

Platanus spp. is a notable avenue and umbrage trees which plays a very important role in the city virescence. However, when it transfigure the city, it's flosses from the crack fruits and hibernacula and pollens spread everywhere, which cause respiration system handicap, anthema allergy and seriously pollute the environment So solving this problem has practical significances definitely. Explants from 2-3 years old wattles were used to establish the fast reproduce and leaves regeneration system. Meanwhile, investigating hormone levels, vitrification and browning effects on the regeneration system to build the genetic transform system via optimizing conditions; Abloom key genes of PtAP3 were genetically transformed in order to find an effective ways to decrease the fruits of Platanus spp. According to the experimental results:Exterior disinfected by 70% ethanol for 30s, then marinated in 0.1 % HgCl₂ for 6 min is most feasibled disinfection. When inducement plumules bourgeoned, MS culture with 6-BA 0.3mg/LN IBA 0.1mg/L can speed up bourgeon distinctively. Increment coefficient reached highest when the plumules were transplanted to the multiplication medium with 6-BA 0.5 mg/LN IBA 0.1mg/L. The leaves of Platanus spp. were used as the materials, plus selecting the sorts and consistencies of the hormones to make sure the differentiation culture mediums, which is MS with 6-BA 2.5mg/L, NAA 0.1mg/L.The differentiation ratio of leaf is 98%; And when the buds grow up to 2cm, transplant to the radication medium of 1/2MS with IBA 0.1mg/L, radication ratio is up to 100%.For browning issue of Platanus spp., 10g/LVc were used to pretreat disinfectant explants, Orthogonal Test was applied. 2g/LAC, 1.5g/LPVP, 0.5mg/L6-BA were put in to the culture. The results show that it can decrease the browning in some way. For vitrification, low concentration 6-BA, 2500-3000Lux of illumination, 0.6%-0.65% of agar can restrain vitrification phenomenons.Genetic transforming system were established according to transformation factors of Platanus spp. 3 days leaves of Platanus spp. were used to be intruded for 10min by (OD₆₀₀=0.6)agriculture bacillus. After 3 days cultivating, plus Kan 20mg/L, Cef 400mg/L, the transgenes plants were selected. Via antibiotic pressure and PCR testing, the specific bands in accord with positive clones were selected. Primary evidence proved that the aimed genes have already combined to the genome of Platanus spp.