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Research On Plant Attractant Of Semanotus Bifasciatus (Motschulsky)

Posted on:2008-02-27Degree:MasterType:Thesis
Country:ChinaCandidate:S L GaoFull Text:PDF
GTID:2143360212488661Subject:Forest Protection
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Semanotus bifasciatus Motsh is a major trunk pest that is endemic in East Asia. and caused tremendous losses in China's forestry resources, which is a serious threat to the protection of old trees. the effect of oriental arborvitae volatile to Semanotus bifasciatus and the mechanisms are the focus of this paper,which can support for the prevention of Semanotus bifasciatus as a theoretical basis.The results of The biological characteristics of Semanotus bifasciatus have showed: 1. The hole of Semanotus bifasciatus associated with a certain temperature.2.The main damages of Semanotus bifasciatus located oriental arborvitae tree trunks, while above 3m parts are not damaged. 3.The Mating numbers of Semanotus bifasciatus are not unique ,and the duration varied due to the different individuals, the average duration is 3.2 minutes . 4. when are flying , they are more sensitive to blue and green; while moved, they are more sensitive to black.The acquisition method of volatile compounds, from different mechanical damage exent of oriental arborvitae, are the dynamic headspace body of improved air circulation Acquisition Act. The operation of thermal desorption cold trap-gas chromatography-mass spectrometry used to analyze the the acquisition of volatile compounds. The results showed : at the different mechanical damage states, the volatile compounds are different. Mycene, α -Terpinene,3-Thujene are existed at the bait wood , and release large numbers of volatile compound from bait wood..EAG experiment (EAG) has showed : 1. Semanotus bifasciatus is most sensitive to Terpinolene, following are pinene and Rohan Chamigrene. The potential reactivities of Semanotus bifasciatus by different compounds are positive correlation in the decimus concentration. 2. making use of the smell of Semanotus germari method gets the different responses of Semanotus bifasciatus to varied concentrations of the compounds. compounds at different concentrations found the different biological activity. Limonene 1% solution Thujopsene 1% solution γ -Terpinene 10% and 1% solution has obvious attraction.The EAG experiments by Mixed monomer have showed : 1. when use two monomers for mixture. β - Pinene mixed with γ - Terpinene, 3-Carene,α-Pinene,Limoneneseparately and γ -Terpinene mixed with 3-Carene, -Pinene separately, which are obvious different. The results of Limonene mixed with γ -Terpinene, α-Pinene separately are not obvious. 2. when use three monomers for mixture, (1). β-Pinene mixed with 3-Carene andγ -Terpinene. (2). α-Pinene mixed with 3-Carene and γ-Terpinene. (3).both are obvious different.Extraction of volatile substances through different methods from oriental arborvitae, which used for the EAG and behavior determination. There are three methods . 1 .the steam distillation. 2.Rotary evaporation distillation, 3.supercritical extraction for the extraction of volatile oil oriental arborvitae, the experiment results showed, 1.the best conditions for the extraction by rotary evaporation are NG:water:ethanol=l:4:4 sawdust under the conditions 50 ° C in temperature, atmospheric pressure 0.1 Pa, extract maximum extraction rate of 90 minutes. The best experiment materials are the just-cut sawdust. Extracts from different extraction methods have been identified : GC-MS Identification of crude extract for a total of 15 species likely to be effective components of the material, the main component of monoterpene compounds Lohan Chamigrene, brain and cypress wood resin acids.Field tests proved that the crude extraction + the monomer are the best trapping, which is the effective range of about 50m and effective time is 20 days. The active ingredients for lure are monoterpenes and sesquiterpenes.
Keywords/Search Tags:Semantus bifasciatus (Motschulsky), volatile, electroantennogram, taxis behavior
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