Study On The Response Of Anoplophora Glabripennis (Motschulsky) To The Volatiles From Maples And Herbivore-induced Signals

Anoplophora glabripennis (Motschulsky) is a wood-boring beetle that caused great losses to the forestry economy of China. In order to study the relationship between host plants and insects and to elaborate the selecting mechanism of Anoplophora glabripennis (Motschusky) to host volatile compounds and the signal transductions induced by feeding-damage. With multidisplinary methods, chemoecology, botany, plant physiology, animal ethological, combined with the Thermal-desorption Cold Trap/Gas Chromatography/Mass Spectrum (TCT-GC/MS), High performance liquid chromatography(HPLC), Supercritical CO₂ fluid extraction (SFE CO₂) technique, "Y"-tube olfactometer and Eletroantennograms (EAG), We determined the composition, concentration of the volatiles from the four maples: Acer negundo L., Acer mono Maxim., Acer truncatum Bunge.and Acer platanoides L., as well as the behavior of Anoplophora glabripennis (Motschusky) and the trends of the concentration of the signal compounds. Meanwhile the interrelationship was established among the constituents and content of volatiles, the insect behavior, and the induced signal transudation.A "Y"-tube olfactometer was used to test the role of volatiles from host plants in host selection by Anoplophora glabripennis. The olfactory responses of Anoplophora glabripennis to different plant odors showed preference for certain host plants: Acer negundo, Acer mono and Acer truncatum. The attraction of Acer negundo and Acer mono were significantly different (p <0.05) and the attraction of Acer negundo to the insect was stronger than to Acer mono. Acer platanoides neither attracted nor repelled the insect. The constituents of volatiles and the relative concentrations were different in the four host species, α-terpinene was attractive to Anoplophora glabripennis, while 3-carene, ocimene, 1-penten-3-ol, trans-2-hexenol and trans-germanylacetone were repellent to Anoplophora glabripennis. In Acer negundo only a-terpinene was detected, a-terpinene, ocimene and trans-2-hexenol were found in Acer mono, 3-carene, ocimene and trans-2-hexenol were detected in Acer truncatum ,and 1-penten-3-ol, trans-2-hexenol and trans-germanylacetone were detected in Acer platanoides. With the increase of the resistibility of the four maples, the amounts of ketones alcohols and aldehydes in the four plants increased, while that of alkanes and esters decreased. This was the first time to study on the volatile semiochemicals regulating A, glabripennis...