Separation And Identification Of Pathogen Of Pasteurellosis From Tiger And Its Pathological Observation

A bacterium isolated from Northeast Tiger was identified as serotype Fg and capsule type A, Pasteurella multocida by morphological, germiculture, biochemical characteristics, and capsular test. The animal regression test showed that all the mice died and had the similar pathologic symptoms with the Northeast Tiger resulting that the strain isolated was strongly pathogenic. The drug test showed that the bacterium was sensitive to neomycin, cefazolin and amkin, but highly resistant to mycillin. Virus particle was not detected through negative staining by electron microscope. CPE was not observed in 3 generations after blind passage. These results can eliminate a possibility of the tiger dying of viruses. All these showed that the Northest Tiger died from highly drug resistant Pasteurella multocida.Pathology detected to organs of the Northest Tiger appears septicaemia, especially heart and lung. Cardiac pericardium, epicardium, and endocardium appears bleeding point and ecchymosis; cardiac muscle appears white necrotic focus; lungs were swollen and congestive. Histopathologic examination showed that Henle's fissures were abound with inflammatory cell infiltrate; part of cardiac muscle fibers broke, degenerated, and the heart appears typical myocarditis symptom; blood capillary of alveolar wall highly stretched and congested; alveolar space...