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The Build Of The System Of Fast Reproduction Of Radix Glycyrrhizae And Primary Reasons Of Effect

Posted on:2008-11-22Degree:MasterType:Thesis
Country:ChinaCandidate:J H ZhangFull Text:PDF
GTID:2143360212997510Subject:Bio-engineering
Abstract/Summary:PDF Full Text Request
the build of the system of fast reproduction of Radix Glycyrrhizae. Using aseptic seedling of spouted licorice seeds as exophyte, and introduce 6-BA 0.2-0.5 mg/L, NAA 0.01-0.025 mg/L into MS culture medium as inducing medium. The substances, 6-BA 0.4-0.15 mg/L, NAA 0.1-0.15 mg/L are used in the culture medium for continue a generation for multiplication. The result of the examination shows that, both culture mediums have the function of speed up the reproduction; the basic generation can reproduce more than 3 times. 60% of rooting proportion is exceeded by using 1/2 MS plus NAA: IAA=0.5: 0.1 mg/L, NAA: IBA=0.5: 0.1 mg/L, and only NAA=1 mg/L and 1/2 MS is mixed into the plain rooting culture medium. The highest rate of rooting proportion is produced while using the plain culture medium with no hormone mixed, the rooting proportion exceeds 80% and over. MS plain culture medium produces the best result of spout of seeds, where it can also strengthen seedlings. After 5 generations of reproduction, the hormone shall be gradually taken out of the culture medium; of MS plain culture medium is used once only to strengthen the seedlings. The vitrified seedlings can be cured (or recovered) into normal seedlings by MS plain culture medium or 1/2 MS plain culture medium. 85% of survival rate can be achieved in transplants by using turfy soil:Pearlite =1: 1.
Keywords/Search Tags:Reproduction
PDF Full Text Request
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