Effect Of Chlorogenic Acid On The Non-Specific Immune Factors And Disease Resistance Of Carassius Auratus Gibelio

The purpose of this experiment is to investigate the influence on the non-specificimmune factors and disease resistance in Carassius auratus gibelio by supplemented withchlorogenic acid in basical diet. The application of this study is to provide more theoreticalevidence for the research of chlorogenic acid in disease prevention of aquatic animal. It alsogives scientific instruction to aquaculture.In the experiment 1, the effect of the chlorogenic acid on non-specific immune factorsin Carassius auratus gibelio was studied. 450 fishes were divided into 6 groups and werefed diet supplemented with different ratio of chlorogenic acid, which was 0 (controlⅠ),0.0025％(experimentⅡ), 0.005％(experimentⅢ), 0.01％(experimentⅣ), 0.02％(experimentⅤ) and 0.04％(experimentⅥ) respectively. At 0, 10, 20 and. 30.days, thenumber of the leucocytes, serum lysozyme activity, serum superoxide dismutase activityand serum alkaline phosphatase activity were monitored. The results showed thatchlorogenic acid had the positive effect of various degrees on the non-specific immunefactors, but the effective time was not synchronous, the number of leucocytes was reachedthe highest by fed diet supplemented with 0.02％chlorogenic acid for 30 days, the numberof leucocytes was 1.894×10~7/ml, and increased by 43.7％compared with the controlgroup(P＜0.05). The serum lysozyme activity and the serum superoxide dismutase activitywere reached the highest by fed diet supplemented with 0.02％chlorogenic acid for 10 days,The lysozyme activity and the superoxide dismutase activity were 5734.4U/ml and239.72U/ml, and increased by 159.7％and 109.9％respectively compared with the controlgroup(P＜0.05). The serum alkaline phosphatase activity was reached the highest by feddiet supplemented with 0.0025％chlorogenic acid for 30 days, the alkaline phosphataseactivity was 9.14U/100ml, and increased by 145.7％compared with the control group(P＜0.05). However, fish were fed diet supplemented with the highest dosage chlorogenic acid(0.04％) with long time, the number of leucocytes would be reduced and the serumalkaline phosphatase activity would be bated.In the experiment 2, the effect of the chlorogenic acid on disease resistance in Carassius auratus gibelio was studied. 300 fishes were divided into 5 groups and were feddiet supplemented with different ratio of chlorogenic acid, which was 0 (controlⅠ, controlⅡ), 0.02％(experimentⅢ, experimentⅣ) and 0.0025％(experimentⅤ)respectively. 10days later, Carassius auratus gibelio in controlⅠand experimentⅢwere challenged withAeromonas hydrophila (5×10~9CFU/ml) for 0.2ml. 30 days later, controlⅡ, experimentⅣandⅤwere challenged. The mortality and the relative percent survival (RPS) of Carassiusauratus gibelio in every group were monitored in 7 days. The results showed that thedisease resistance of experimentⅢwere significantly higher than the controlⅠ, themortality of controlⅠwas 60％, the mortality of experimentⅢwas 23.3％, and the relativepercent survival was 61.2％. The disease resistance of experimentⅢandⅤwere notsignificantly improved.Above all, the chlorogenic acid could enhance the immune competence and diseaseresistance of aquatic animal, most effectively that Carassius auratus gibelio fed dietsupplemented with 0.02％chlorogenic acid for 10 days.