| 'Meiren Mei',Rosaceae,Prunus mume,is the hybrid progeny of 'Gongfen Mei'(P.murae) and Myrobalan Plum(P cerasifera),shrub or abor,'Meiren Mei' inherits Myrobalan Plum's red leaves and characteristics of'Gongfen Mei':pink flower,easy blooming,vigorous,dense fowers and low temperature resistant.Because both leaves and flowers are of ornamental value and it has a strong stress resistance,'Meiren Mei'bacame an important specie of Prunus mume.It could be widely used for gardening especially north China.In this article,we discussed following factors affecting the micropropagation of 'Prunus mume' Meiren Mei' including disinfection,CTks,auxins,GA,basic medium,carbon resource,pH , culture method,Fe-EDTA,etc.The effective way for micropropagation of Prunus mume 'Meiren Mei' was tentatively explored.The main results are as the following:1.Disinfection ofexplant:Spring buds were cut into 0.5-1cm segments,phloem and squama were peeled,rinsd in water for 24 hours, sterilized with 70％alcohol and 0.1％HgCl2,then rinse in sterile water.The Browning rate increases when sterilize time increases.The best combination is 70％alcohol 30s and 0.1％HgCl2 10min.Browning rate and disinfection rate are 4.7％and 37.5％.2.Adventitious buds inducement cultureEffect of different concentrations of BA,KT,GA3 on inducement culture of 'Meiren Mei' were studied.WPM + BA 1.0mg/L + NAA 0.1mg/L + Glucouse 3％+ agar 0.7％is the best medium for adventitious buds inducement,explants were cultured under light intensity of 16001ux,25+2℃,photoperiod 16 hours/day.The inducement rate and average regenerated shoots are 88％and 1.47.0.5-1.0mg/L GA3 had no effect on inducement and elongation of the buds.3.Multiplication culture:Effects of basic mudium,carbon sourses,CTKs and auxins on multiplication culture of were studied.WPM was the best basic medium for multiplication culture of 'Meiren Mei', 3% glucose was better than 3% sucrose. Auxin can improve the elongation growth of shoots, 0.1mg/L NAA is better than IBA, the Vitrivication rate increase when the concentration of NAA increase, 0.5mg/L 6-BA or 0.4-0.6mg/L TDZ is best plant growth regulation concentration, alternation of high CTK concentration of 1.0 6-BA or 0.8mg/LTDZ after several low concentration of CTKs culture could promote the multiplication rate while reducing vitrification shoots.4. Rootig culture:Adding 0.2mmol/L Fe-EDTA to multiplication medium promote the content of chlorophyll and photosynthesis, benefit to shooting. Modified WPM was the best basic medium, 3% glucose was better than other concentration and sucrose. When shoots were rooted in liquid medium, the roots were more than those in solid medium, but the shoot were easy to become virtrification and callus was induced in the surface of the roots.Both IBA and NAA were positive in rooting culture of 'Meiren Mei', NAA had a better inducement rate than IBA at the same concentration. Roots are rough in the surface when shoots were rooting in medium with auxin,especially when the concentration was1.0mg/L, the roots were not connected with shoot tightly.A two step rooting method was used for P. mume 'MeirenMei', shoots were first cultured in WPM + 1.0mg/L NAA for 7-10days and than transfer to the same medium without NAA.
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