Analysis Characteristics Of Physio-Biochemical And Anti-Low-temperature Sweetening Of Transgenic Ptato (Solanum Tuberosum L.) With Antisense AcInv Gene

The potato (Solanum tuberosum L. ) is an world-wide planting crop plant serving as one of the important source of food and industrial raw material.However, for the reason of the time gap between harvesting and processing. Therefore, a period of storage is often needed. Potato tubers are generally storaged at low temperature in order to reduce pesticide hazards, embryonic and respiratory consumption. However, reducing suger accumulated in potato tubers will be increased during storage at low temperature. It seriously affects appearance quality and edible quality of the fried potato processing products. Therefore, It is very important to cultivate new lines which is resistant to cold induced sweetening to meet the need for processing industry and Annual supply of the potato. The objective of this study was to investigate the characteristics of physiological and biochemical in growing, and to detecte tolerance to cold induced sweetening and qualities of potato tubers in the transgenic lines of potato of Atlantic, Cardinal,and Favorita. The main results were showed as follows:The physiological and biochemical analysis showed that the chlorophyll content of Anti-Aclnv Atlantic were generally higher than that of the parental line served as control, and the chlorophyll content of two generation Anti-Aclnv Atlantic was significant higher than that of the control in the flowering period and tubers. In general, The SOD activitity, POD activity and CAT activitiy of the four lines of transgenic potato with Antisense AcInv were significantly increased compared to the control corresponding parental lines. However, The malondiadeyde content and relative conductivity were distributed slightly different throughout the samples . The analysis of bands of Peroxidase isozyme also indicated that the species lines had significant variations between the transformed Aclnv antisense gene potatoes and control receptor.The dry matter content of transgenic tubers remainded constantly or varied slightly with the increasing age at low temperature storage period and, the total starch and the amylopect was decreased gradually, on the other hand, the proportions of amylose and amylopectin in the transgenic lines were increased but lower than that of the non-tansgentic lines. On the whole, the variation of starch content were not significant in different generation such as C1 and C2.With the extension of storage time,the tuber reducing sugar content of tested material was increased gradually. Apart from anti-aclnv cardina had a reducing sugar content similar to parental line after 30 to 60-day storage, the reducing sugar content in transgenic lines of the other two varieties significantly lower than parental lines. There were no significant difference in reducing sugar content between C1 and C2 during cold storage and the variation was less than 0.1%. The increased tuber reducing sugars content of transgenic lines were lower than that of control lines and showed a significant resistance cold induced sweetening.