Biology Character And Biocontrol Character Of T.viride

The T.viride is a kind of distribution widespread soil custom occupies the fungus, familiar exist in plant cadaver and animal excrement. Besides individually for the weak pathogen germ, the majority has to the control for fruit pathogen. Because of the T.viride universality,oppresses diversity and parasites universality, it is one kind that has the very potential bio prevention fungus.The T.viride biology characteristic,antagonistic characteristic;Ferment condition had been studied,The results showed as below:1.The spore sprouting rate achieves 92.1%; the adaptation time is 1.5d , the logarithm increases:1.5-3.5d , the balanced time is 3.5-6d and 6-8d of declines time; the basic culture medium is the best; The temperature is remarkable for the mycelium and spores; It is growth in pH 2-11, the mycelium growth is the quickest in pH 5-6, the spore has the biggest quantity at pH 7; The light has no obvious to mycelium, but obviously avails to the spore production; The glucose and maltose are the best carbon for the spore and mycelium; asparagines is the best nitrogen for the spore and mycelium; The different vitamins have the different function to the spore and mycelium; The trace elements have the promoter action to the mycelium, Mn+2 is the most advantageous to the spore.2.The T.viride's parasites resistance was studied using ten kind of pathogen germs that had been separated in the laboratory, It is discovered that other's pathogen germs were remarkable suppressed besides Rhizopus stoloniferr and Colletotrichum pawpawIt is certain which is mostly for parasitism and secretion control way by above eight kind of pathogen germs that had been achieved the remarkable suppression were carried on the anti-experiment by T.viride metabolite. It is discovered that only Aspergillus. niger and Colletotrictum gloeos were suppressed by the metabolite and remarkably, other are not remarkable all.For further seeks the control mechanism, cell membrane,mitochondria,karyon,cytoplasm and so on of Aspergillus niger that has been treated by T.viride metabolized distorted withered by observing transmission electron microscope .Vacuole of Colletotrictum gloeos mycelium had expanded distorted ,the color of mitochondria and chloroplast had changed ,and decomposed the granule, karyon had distorted , cytoplasm had turned into colloid, Vacuole,mitochondria and chloroplast of Colletotrictum gloeos organelle had been remarkable restrained3.Finally, through preliminary analyzing the liquid fermentation and fermentation fluid, best culture medium: wheat bran 4.5%, apple marc 2.0%, banana skin 2.0%, KH2PO4 0.30‰, MgSO4 0.18‰, CaCO3 0.5‰, NaNO3 2‰; the best raising condition: temperature 28-20-30℃, mixing speed 125r/min, pH6, time4d, inoculability quantity 8%。...