| Methionine is one of animal essential amino acids,and ruminant restriction amino acid.Except synthesizing protein,participating many transfer-methylic reactions and producing many containing methylic physiological active materials.About mono-stomach animal,to increase methionine amount in diet can satisfy its methionine requirement. However,due to ruminal microorganism degradation,to increase methionine amount in ruminant diet can't satisfy its methionine requirement.And ionophore can inhibit microorganism deamination of protein in rumen,which decreases protein degradated in rumen.However,it hasn't been reported whether ionophore can inhabit amino acids' deamination or not.So objective of this thesis to study on inhibiting effect of ionophore on ruminal metabolism of methionine,to increase methionine by-pass amount and come true a new method of rumen protection.In Exp.1 effect of ionophore on methionine metabolism of runen.3 goats fitted with fistula in the rumen.Experiment included 4 periods.Every period which was a treatment was 6 days.The treatments in the experiment were based diet plus 0(Control group,CG),15g Met(Treatment 1,T1),15g Met and 0.15g lasalocid(Treatment 2,T2),15g Met and 0.20g lasalocid(Treatment 3,T3).Sampling time was at 0 hour(feeding before morning),2 hours(feeding after morning),and 5 hours(feeding after morning)in the last day of every period.The content of free methionine(FM)and related enzymatic active in samples treated were determined.In 0 hour GOT active in serum sample of T2 was significantly lower than that of T1(P＜0.01);GGT active in rumen fluid sample of T3 was significantly lower than that of T1(P＜0.05),GOT active in rumen fluid sample of T2 was significantly lower than that of T1(P＜0.05),and GOT active in rumen fluid sample of T1 was significantly higher than that of T3(P＜0.01).In 2 hours,GGT active in serum sample of T2 was significantly lower than that of T1(P＜0.01);GGT,GOT and GPT actives in rumen fluid sample of T3 was significantly lower than that of T1(P＜0.01),GOT and GPT actives in rumen fluid sample of T2 was significantly lower than that of T1(P＜0.05),the content of RFFM in rumen fluid sample of T2 and T3 was increased by 36.4%and 36.6% Compared T1.In 5 hours,GGT active in serum sample of T2 was lower than that of T1 (P＜0.05);GPT active in rumen fluid sample of T2 and T3 was lower than that of T1 (P＜0.01),the content of RFFM in rumen fluid sample of T2 and T3 was higher than that of T1(P＜0.05),the content of RFFM in rumen fluid sample of T3 was increased by 10.6% Compared T2.We concluded that lasalocid had invariably effective in inhibiting GGT and GOT actives in serum and rumen fluid and GPT active in rumen fluid,lasalocid can increase ruminal stability of methionine,and effective of T3 was better than that of T2.In Exp.2 effect of rumen stabilizing agent on beef cattle performance.This experiment adopting single factor random blocks design,9 Charolais steers (315kg±24.5kg)were randomly divided into three groups(control group,CG;test 1 group, T1;test 2 group,T2),three repeats in every group,one steer in every repeats.The test groups in the experiment were based diet plus 0.15%Met(Control group,CG),0.13% Rumen stabilizing agent(RSA)and 0.15%Met(Treatment 1,T1),0.13%RSA and 0.20% Met(Treatment 2,T2).Pretesting time was ten days;the experiment was divided into three phases,twenty-five of every phase.The results showed that Feed efficiency(FE)and daily gain(DG)of T1 and T2 in the third phase 0.1%,4.3%and 5.6%,10.5%,respectively. FE of T2 in the third phase was higher than that of T1 and CG for 9.3%and 28.5%, respectively.DG of T2 in the third phase was higher than that of T1 and CG for 10.5%and 40.0%,respectively.DG of T1 was higher than that of CG for 26.7%.FE of T1 was higher than that of CG for 21.2%.Thus,we concluded that RSA had invariably effective in promoting DG and FE of beef cattle,which had related with action time and the quantity of Met in diet.
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