Preparation And Antioxidant Activities Of Bioactive Peptides From Bee Collected Rape Pollen

Bee collected rape pollen (BCRP) is a natural product with high nutritional value due to containing considerable amount of proteins and functional components. More and more consumers and researchers are interested in BCRP because it is able to boost immunity system of human. However, high antigenicity and astringent taste of BCRP limits its application in human diet.Proteins approximately account for 30% (w/w) of BCRP and are very difficult to be completely extracted due the interaction with other components. Three different proteases were used to extract the protein in this research to reach a standard hydrolysis degree. Alkaline protease was selected due to best hydrolysis effect. The enzymatic hydrolysates were separated into 4 different portion based on their molecular weight range by membrane filtration, i.g. 200Da～1000Da,1000Da～5000Da,5000Da～10000Da and above 10000Da. The antioxidant activities of these samples was evaluated bu three antioxidant models in vitro, namely hydroxyl radical scavenging, superoxide anion radical scavenging and and anti-peroxidation of lipid. It was found that the antioxidant activities of enzymatic hydrolysates were better than that of water exteact of BCRP, and microfiltration membrane was an effect way to improve the antioxidant activity of the enzymatic hydrolysates.