| Acer davidii Franch, which belongs to genus Acer,family Aceraceae, is 8-12meters high.Thebark is green with the stripes. Because it's beautiful and suitable for sightseeing, the futureimplication is great. It is wildly applied in park-green engineering, therefore,it has an extensiveprospect.At present, more and more people begin to pay attentionto it but simple introduction ofits shape and the research of its resistant ability has been only made, however, the organization ofits culture doesn't be reported. The paper has a comprehensive and thorough research from twosides of the inductive rate of adventitious buds, with explant of Acer davidii Franch and leaf,unmature embryo.The result is as following:1. The effect of different disinfectors and treatments time on David Maple's stems with budswas studied. The results showed that HgCl2 is more effective than NaClO. There are nosignificantly difference on explant survival rate between 0.1％HgCl2 for 7 min and 0.2％HgCl2 for 5 or 7 min. After treated with 75％alcohol for 60 s and followed by 0.1％HgCl2 for 7min, the survival rate and the Contamination rate were 1.9％and 90.4％respectively. From the middle April to July, the explant survival rate were more than 90％,it is the suitable dates to take materials.The pollution of embryo will be 0 in the solutionof 60s+0.1％HgCl2 for 3 min.2. The stem leaved later in the medium with IBA than NAA in it.And the leaf rate of thestem in the medium with IBA in it is only about 70％;but the stem in the medium withNAA which leaf rate is,89％,and the buds come out within 7～9days,while the whole plantgrow better than in the medium with IBA. The best culture is MS+6-BA0.5 mg/L+NAA0.10 mg/L, sucrose 30 g/L, and leaf rate is 89％,higher than other solution evidently.3. Basal medium MS with high concentration of salt iron is more suitable for stem segmentinitial and proliferation culture than other low salt medium such as WPM and 1/2 MS.4. Multiplication rate of the stem in the medium with 1.0mg/L 6BA in it is 63％,and themultiplication coefficient is 2.5,which is better than KT in it; the medium with 1.0mg/L6BA and 0.05～0.1mg/L NAA in it can improve bud to be induced; the medium with GA30.5～1.0 mg/L is helpful for the multiplication and elongate of shoot moreover shoots arestrong and in full vigor.The optimum medium for proliferation are concluded by L9 (33)orthogonal experiment. The best proliferation medium was MS+6-BA1.2mg/l+NAA0.12 mg/l+GA30.7mg/l +sucrose 30g/L, in which the shoots can growformally.The temperature between (25±2)℃is good for the growth of medium 5. The quality of the root system better on MS medium with IBA0.3 mg/L,but the generalquality is lower. The root is only 2～3,the main root is too long without subroot.And theSurvival rate is low when transplanting,6. The quality of callus will not different significantly.the inducing rate and growth speedof callus were heightened by 15 days-dark culture firstly and then days-light culture.7. Different concentration of 6-BA+NAA+2, 4-D will get according to the experimentL9(33). On the callus of inducing medium, although the rate can reach 100％,the inducingrate of the shoot is especially low, with only some single shoot appeared. The shoot growthickly, but the shoot induced by callus grow difficult and slowly and the rate is low.8. Root rate was maximized(80％)for handwood cutings of half wooded stem treated with0.8mg/L NAA for 30 min and the the average number of root can reach 4.
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