Study On Tissue Culture And Rooting Physiology Of Acer Yangjuechi

Acer yangjuechi Fang et P.L.Chiu is a deciduous species belongs to the genus Acer Linn..It is a kind of species with tall and straight trunk and beautiful posture.The samara of the tree is special and fantastic which looks like the angle of sheep.It is a kind of garden greening plant with great ornamental value.Furthermore,as a unique and ancient relic plant of China,A.yangjuechi has important scientific value in plant geography and paleobotany.The paper carried out callus induction,proliferation and differentiation in tissue culture and rooting physiology during cutting propagation taking leaves,stems and branches of A.yangjuechi as materials,to provide theoretical guidance and technical support for its preservation and utilization of the reproductive technology system and elite germplasm resources.The main conclusions were as follows:(1)The experiment was conducted to induce culture of explants in April and June of 2017,results showed that the most suitable time for the callus induction is in April,at this time,the callus grows rapidly with high callus induction rate and low pollution rate.According to induction rate and pollution rate after inoculation,the best way of explant sterilization was 75% alcohol(30s)+ 0.1% Hg C12(10min).(2)Three types of basic medium-1/2MS?MS and WPM-were chosen to induce callus,WPM medium has the best effect on induction of the leaf and stem,with induction rate of 33.1% and 71.0%.In the selection of explant type,the induction effect of stem segment was better than that of leaf blade.In the stem segment,the granule of the callus was larger and more injured,when that of the leaf blade was smaller and could not be blocked.(3)Used L9(3)4 orthogonal experiment design on induction culture,the basic medium type had the greatest influence,secondly it was 6-BA,thirdly was NAA,KT had the smallest influence.The optimum medium formulation was WPM + 0.3 mg·L-1 NAA + 0.5 mg·L-1 KT + 0.5 mg·L-1 6-BA + 30 g/L sucrose + 7 g/L AGAR powder.The induction rate was 69.20% for stem segment and 41.67% for leaf blade.(4)On the multiplication culture stage,the optimum medium formulation was WPM + 1.0 mg·L-1 6-BA + 0.1 mg·L-1 NAA + 30 g/L sucrose + 7 g/L AGAR powder,the multiplication coefficient was 2.40.(5)There were no obvious adventitious buds in callus during differentiation culture,there were light green or white green shoots on the surface of callus.According to the growth situation of callus in different differentiation medium.More adventitious bud points were produced in callus in the treatment group with higher TDZ quality concentration.It can be seen that TDZ can promote the differentiation of callus.(6)There was no root formation in the cuttings of different growth matrix and growth regulating substance treatment group,however,in the cutting process,the contents of nutrients and active enzymes in the cuttings changed correspondingly;During the cutting process,the nutrients of soluble sugar and soluble protein in the cuttings were consumed,and the nutrient consumption rate of the GGR treatment group was greater than that of the control group;The activity changes of POD,IAAO and SOD were closely related to rooting,and the process of growth regulating substance could accelerate the change of enzyme activity content in the cuttings.