Effect Of Different Site Types On Growth And Development Of Siraitia Grosvenorii Tissue Cultured Seedlings

With outstanding characteristics such as devirus, premature, high yield etc, tissue cultured seedlings from Siraitia grosvenorii were widely cultivated. But existing a widespread loss of target in the spreading and application becauce there is no scientific thinking in environmental and regional adaptability. Local ecology of 3 site types were measured and the same species Siraitia grosvenorii plants were used as material in this experient to discuss the effect of different site types on growth and development of tissue cultured seedlings from Siraitia grosvenorii By ecological factor. Which can offer reliable evidence for cropping of Siraitia grosvenorii with tissue cultured seedlings in different varieties type.The experient was carried out in 2006 in Lingui of Guangxi. We divided the study plot into three groups: Hilly region, Flat terrain and Mountainous area. The 3 Siraitia grosvenorii ecological districts were located in the two adjacent villages and towns in Lingui of Guangxi:WuTong and ChaDong. Siraitia grosvenorii variety trialed in this experient is the same:QingPi. These Siraitia grosvenorii seedlings from tissue–clture were planted in late April 2006. The measures of culture and management were the same. The chiefly results were as follows:1. The 3 Siraitia grosvenorii study plots are all acid soils. Excep study plot in Mountainous area lower layer of soil(20-50cm)is sandy loam, Others are all loam.2. Soil nutrient and soil enzymeactivity is remarkably correlated with yield of Siraitia grosvenorii. The soil enzyme activity in higher potency strains is notably higher than that of in lower potency strains.3. The ground temperature of study plot in Flat terrainis the highest and study plot inMountainous area is the lowest among the 3 Siraitia grosvenorii ecological districts in the same month. Basically, the ground temperature descends with the increase of mountain elevation.4. The same variation trend of air temperature was present in growth periods of plants in each study plot: the temperature gradually rises from May to July and decreases gradually from July to September. The air temperature of study plot B(Flat terrain)is the highest and study plot C(Mountainous area)is the lowest among the 3 Siraitia grosvenorii ecological districts in the same month.5. Relative humidity of each study plot gradually rises from May to August and decreases gradually from August to October. From May to July at the same stage of every day,relative humidity followed the order: Mountainous area>Hilly region>Flat terrain and the order in relative humidity Was Hilly region>Flat terrain>Mountainous area at the same stage of every day From September to October.6. Content of total N, total P and total K in plant and content of chlorophyll in leaves were measured dynamically in the study plot of each group. It was found that Content of total N, total P in plant followed the order: seedling stage>flowering stage>picking date and the order in content of total K was picking date>flowering stage>seedling stage; The variation regularity showed that content of chlorophyll gradually rises from seedling stage to flowering stage till the Maximum was shown, and then started to decrease. Content of chlorophyll in seedling stage followed the order: Hilly region>Flat terrain>Mountainous area and the order in content of chlorophyll in flowering stage and picking date were Flat terrain>Hilly region>Mountainous area among the 3 Siraitia grosvenorii ecological districts in the same growth period.7. The measuring results with diurnal variations of photosynthesis in Siraitia grosvenorii leaves show that the net photo-synthetic rate displays a significant middy depression at noon. Diurnal variations show a double peaks curve. Net photosynthetic rate of leaves rises with the increase of light intensity till it become the biggest increment that is Pn at light saturation point (Pmax). The net photosynthetic rate of Siraitia grosvenorii leaves in different site types followed the order: Hilly region>Flat terrain>Mountainous area at the same stage of every day.8. It was found that phenophase of Siraitia grosvenorii in Hilly region study plot was the first and Mountainous areais study plot the latest among the 3 Siraitia grosvenorii ecological districts by observation and comparison.9. The per plant yield of Siraitia grosvenorii followed the order: Hilly region>Flat terrain>Mountainous area in the 3 study plots, That is to say, the per plant yield of study Flat terrainis plot the highest and that ot Mountainous areais study plot the lowest among the 3 Siraitia grosvenorii ecological districts.10. The content of total saponin in tissue-cultured seeding of Siraitia grosvenorii in Mountainous are study plot ais the highest, and the content of that in Hilly region study plot and Flat terrain study plot have no remarkable differences. There were no remarkable differences in the content of vitamine C in fresh berries among the 3 Siraitia grosvenorii ecological districts.The experiment results show that effection of climatic factors and terrain fastorson the growth and development of Siraitia grosvenorii with tissue cultured seedlings were the most important. The proper ecological environment could increase yield and quality to Siraitia grosvenorii with tissue cultured seedlings. Compared to the other site types, hilly region is most helpful to increasing yield of Siraitia grosvenorii fruits and yet mountainous area is the beneficial to improving quality of Siraitia grosvenorii fruits.