Study On Candidate Gene For Fecundity In Cele Black Sheep And Duolang Sheep

BMPR-IB gene as a fecundity candidate gene was studied in Cele black sheep(167 blood samples) and Duolang sheep(509) by Tetra-primer ARMS-PCR technique. The results showed that there was a mutation (A746G) of BMPR-IB gene in Cele black sheep and Duolang sheep, between them, the BB,B+ and ++ frequence in Cele black sheep were 0.10,0.35 and 0.55 respectively. There was no BB genetype in detected Duolang sheep, and the B+,++ frequence were 0.06 and 0.94 respectively. Analyzing the association with litter size showed that, Cele black sheep with genotype BB had a most significantly difference litter size than those with genotype ++ (P<0.01); B+ had a significantly difference litter size than those with genotype ++ (P<0.05); BMPR-IB gene was a major gene influencing prolificacy in Cele black sheep; the Tetra-primer ARMS-PCR technique was simple,rapid and economical for detecting BMPR-IB mutation and other SNP locus.BMP15 and GDF9 as candidate gene of high fecundity were detected in Cele black sheep and Duolang sheep.No mutant genetypes were identified in FecG H lacus of GDF9 and FecXG,FecXB,FecXI,FecXH four locus of BMP15 .Our results suggested that BMP15 and GDF9 may not be the major gene for the high prolificity of Cele Black sheep and Duolang sheep.ESR gene as a fecundity candidate gene was studied in Cele black sheep(44 blood samples), Duolang sheep(176), Hu sheep (31) and Small Tail Han sheep (33blood samples) by PCR-RFLP and PCR-SSCP technique. The results showed that there was a mutation (C363G) of exon 1 of ESR gene in Cele black sheep, Hu sheep and Small Tail Han sheep . Among them, the EE,E+ and ++ frequence in Cele black sheep were 0.03,0.31 and 0.66 respectively; the EE,E+ and ++ frequence in Hu sheep were 0.03,0.19 and 0.77 respectively. And the EE,E+ and ++ frequence in Small Tail Han sheep were 0.03,0.42 and 0.55 respectively. Analyzing the association with litter size showed that, Cele black sheep with genotype EE had a most significantly difference litter size than those with genotype ++ (P<0.05); ESR gene was a major gene influencing prolificacy in Cele black sheep.