1. Databases Of Large Economic Seaweeds From The South China Sea 2. Studies On The Chemical Constituents And The Antiviral Activities Of Petroleun Ether Fraction From Ardisia Chinensis Benth

The construction of databases in large economic seaweeds from the South ChinaSea and the chemical constituents and antiviral activities of petroleun ether fractionfrom Ardisia chinensis Benth were studied.The databases of large economic seaweeds from the South China Sea wereconstructed by field investigation, collection and arrangement more than 60 seaweedsresearch data, the web address was: http://course.jnu.edu.cn/sky/haizao/. Databaseswas designed by ASP+ACCESS, the database query of databases was included ofhome, summary, resource, information search, BBS, scientific research project andcorrelated links. In the seven contents, the seaweeds resource was the key in thewhole databases, which contained twelve aspects, such as chemical constituents,bioactivities, nutrient components, application development and reference and so on.The research data of was integrated, abundant. The background management ofdatabases was entered, modified and deleted data or photos. The databases were firstbuilt in seaweeds at home and abroad, which were based on the research works for theseaweeds, it had special function and meaning for promoting high value of theseaweeds.The database provided original experiment data: nutrient components of anumber of seaweeds from South China Sea were analyzed and evaluated.Ⅰ. Fatty acids compositions and contents of sixteen seaweeds were determinedby GC-MS. The result indicated that the content of C_16:0 was higher than othersaturated fatty acids; the most of Rhodophyta and Phaeophyta were contained C_15:0and C_17:0, the two kinds odd number fatty acids were seldom seen on the terrestrialplants; Phaeophyta and Chlorophyta were rich in polyunsaturated fatty acids (PUFAs),among them, the content of C_18:2 and C_20:4 of Ishige okamurae was the highest, being22.35% and 10.76%, Codium fragile contained high level of C_18:3, the content was16.26%. Ⅱ. Nineteen elements in seaweeds were determined by inductively coupledplasma atomic emission spectrometry (ICP-AES). The relative contents of constantelements Mg, Ca, P, K and trace elements Fe,Mn,Zn were higher than the other ones.In these three poisonous elements, the content of As was higher than Cd, Pb.Ⅲ. Seventeen kinds of amino acid compositions and contents were determinedby acid hydrolyzed. The content of amino acid was about 4.40%～21.75%, and thecontent of essential amino acid (EAA) occupied 32.74%～49.21% of the total aminoacid (TAA). The higher content was contained Asparic acid, Glutamic acid, Alanine,Leucine and Valine; and the poorer content was contained Cysteine and Histidine.Ⅳ. Fat was determined by Soxhlet extraction; Fiber was tested by acidicdetergent; protein was measured by Kjeldahl's method; Moisture was determined bydesiccation method at atmospheric; Ash was tested by burning method. The contentsof them were 0.09%～5.83%, 1.70%～43.52%, 4.08%～23.70%, 5.48%～19.72%,5.02%～35.10% and 6.68%～56.21% respectively.Ⅴ. The ethanol extract from seaweeds were analyzed by IR spectra. Thespectrum had strong repeatability and comparability, which was important supplementfor traditional identification of external morphology.The results showed that seaweeds contained a plenty of fatty acids and aminoacids; rich in minerals elements, fiber and protein which all were required by body.These provided the basis of nutrient evaluation for development of seaweeds food.The antivirus activities of ethanol extracts and aqueous extracts from seaweedswere tested by cytopathic effect reduction assay and MTT assay. As a result, theextracts and fractions exhibited anti-Cox B3 virus activity stronger than anti-HSV-1virus activity, the ethanol extracts had strong cytotoxic activities for HeLa and Verocells, while Sargassum pallidum possessed the most potent anti-Cox B3 virus activityand anti-HSV-1 virus activity; Notably, aqueous extracts showed antiviral activitiesagainst Cox B3 virus and HSV-1 virus to different extent, Eucheuma striatum andCaulerpa racemosa var peltata exgibited more potent anti-Cox B3 virus activity and anti-HSV-1 virus activity, all these suggested that the antiviral constituents inseaweeds maybe its polysaccharide. On the basis of antivirus activities data, antitumoractivities were discussed, the method was chose different polar solvents to extract,and compared antivirus activities of seaweeds extracts under different solvents.The chemical constituents of petroleun ether fraction of Ardisia chinensis wereisolated. Their structures were elucidated by spectroscopic analysis and comparisonwith published data. Two groups compounds were obtained and characterized. Theywere:①.Cholesta-5,23-dien-methanol, (3β,5a)-Cholesta-8,24-dien-3β-oxy, stigma-sterol;②. (3β-lanosta-8, 24-dien-3-yl)-oxy,β-amyrin, [(3β)-lanosta-7,9(11), 24-trien-3-yl]-oxy. The result of anti-Cox B3 and anti-HSV-1 virus indicated that none ofthem showed obvious antiviral activities.