| A filamentous virus was isolated from Angelica sinensis (Angelica sinensis(Oliv.)Diels). which showing mosaic symptoms in Minxian County, Gansu province in China. Based on morphology and molecular biology properties, it was assigned to the genus Potyvirus, family Potyviridae. Electron microscopic observations of plants showing symptoms revealed the presence of filamentous particles and pinwheellike structures characteristic of the Potyviridae family. The virus is a flexuous rod-shaped particle about 750nm in length and 12nm in width. Its coat protein (CP) has high similarity with other six potyviruses by peptide mass fingerprinting (PMF) analysis. After reverse transcription and polymerase chain reaction (RT-PCR) test with primers designed according to PMF, the 3' terminal 919bp nucleotides was determined, covering partial CP gene and 3'-untranslated region. In sequence comparisons and phylogenetic analysis, the new isolate is closely related to Japanese hornwort mosaic virus (JHMV), Konjak mosaic virus (KoMV), and Zantedeschia mosaic virus (ZaMV). The most closely incompletely related virus is JHMV03 (AB251346). They have 96.59 % and 87.60% identities in the amino acid and nucleotide sequences respectively. These results suggest that the presence of a new member of potyvirus tentatively named Dang Gui strain of Japanese hornwort mosaic virus (JHMV-DG) in Angelica sinensis. And the antiserum against the CP of JHMV-DG has also been prepared in our research.
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