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The Effect Of Salt On Eggplant And The Utilization Of Strain DW1 With ACC Deaminase Activity To Improve The Growth Of Eggplant On Saline Soil

Posted on:2008-07-07Degree:MasterType:Thesis
Country:ChinaCandidate:C LiuFull Text:PDF
GTID:2143360215959620Subject:Microorganisms
Abstract/Summary:PDF Full Text Request
The study was undertaken to look for solutions to solve the salt stress existed in the applied of coastal soil. Eggplant was used to study their physiological and biochemical response to NaCl while the activities of the enzymes from rhizosphere soil were tested. Several strains of plant growth-promoting bacteria (PGPR) that have 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity were isolated from soil samples taken from shangyu city. The effects of the bacterial DW1 on the egglplant were analyzed. The results are summarized as follows:1,The influences of NaCl on the soil enzymes in close proximity to plant root were studied. It was found that the influences of NaCl varied on different egglplant species. The results showed that the activity of urease, catalase and sucrace were strongly decreased with increasing NaCl concentration (0-0.3%). Regressive analyses showed that soil salinity had a binomial relationship with the activity of catalase.2,A bacterium, isolated from saline soil in Shangyu and named strain DW1 could utilize 1-aminocyclopropane-l-carboxylate (ACC) as a sole source of nitrogen. Based on the results of phenotypic features, phylogenetic similarity of 16S rDNA gene sequences, DNA G+C content, strain DW1 was identified as Pseudomonas sp.3,The NaCl content to some extent could promote the growth of the egglplant. The results showed that both of fresh weight and relative water content (RWC) were decreased as the increase of the salt concentration while the content of Na+ and relative electron content (REC) were increased. When the salinity were 0.2% and 0.3%, the content of proline were increase by 319% and 589% comparead with the blank respectively. The uptake of Ca2+ was inhibited by NaCl.4,The strain was capable of promoting egglplant germination percentage and seedling root elongation in the presence of high salt concentrations. Monitoring the response of egglplant plants to a range of salt concentrations in the presence and absence of the bacterium permits an assessment of plant responses to salt. Interestingly, the strain DW1 significantly increased the fresh weights and RWC, while decreased the REC and the content of proline. Egglplant plants showed a substantial tolerance to salt stress.
Keywords/Search Tags:egglplant, salt stress, soil enzyme, ACC deaminase, rhizosphere microbacteria, coastal saline soil
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