Investigation On Tissue Culture Of Ilex Crenata

Ilex crenata, a colored-leaf tree, is widely used in the landscape. This study used newtip stem section of Ilex crenata as explant, and determined effects of various factors ontissue culture of this species. The main results and conclusions in this paper are listed asfollows:1 Different kinds of basic medium and hormone matching were applied for thepurpose of examining their effects on callus induction and differentiation. It was found thatthe new tip terminal bud was the optimum explant. In addition, when the sterilizationprotocol was 40 second treatment with 75% ethylalcohol followed by 3 min treatment with0.1% HgCl₂, the rates of callus induction and differentiation were much high. Furthermore,when the sugar concentration was 3% in the medium of MS supplemented withKT1.0mg·L^-1, NAA0.2mg·L^-1, the rate of callus induction reached the highest value.2 We also investigated the effects of hormone, agar, pH and light on calluse subcultureof Ilex crenata. The result showed that calli grew very well and calli proliferation was alsovery ideal in the MS medium supplemented with 6-BA 1.0 mg·L^-1, NAA 0.5 mg·L^-1, 0.2%agar under pH6.0 and 15001x light condition.3 When the shoots regenerated from calli were transferred to root medium, the number,diameter and length of roots were respectively determined. Additionally, we investigatedimpacts of types and concentration of hormones, E material density and different kinds ofbasic medium on root regeneration. The result demonstrated that 1/2MS mediumsupplemented with 0.3mg·L^-1 NAA was most suitable for root regeneration and thesupplement with 0.1% activated charcoal had no significant effects on root regeneration ofIlex crenata.4 We also carried out experiments to determine effects of medium, acclimationmethods on the growth of Ilex crenata plantlets. The result showed that the optimummedium consisted of a 5:1:4 [volume ratio] mixture of charcoal of grass: ashes of the ricechaff: perlite. The survival rate of plantlets was relatively high when plantlets were plantedin the mixture of vermiculite and perlite or peat and perlite. River sand can also be used asmedium just because of low cost, but the survival rate of plantlets and plant height weresignificantly lower in comparison with optimum medium.