Study On The Technique System Of Tissue Culture Of Phalaenopsis

To explore the efficient way in vitro culture,the cutting segments of flower-stem-knot were utilized to induce clustered shoots to multiply.Experiment was designed primarily by single factor and orthogonal design.Through variance analysis and multiple comparisons, we found out a series of technical parameter combination for the tissue culture of Phalaenopsis,which provided the scientifical and technical support for its factorization production.The main results were as follows:1.The best type of explants in vitro was the cutting segments of flower-mid-stem-knot which flowered.The pretreated explants immersed in 0.1%HgCl₂ for 13 minutes was sterilize well.The treatment of 6-BA 10 mg/L+NAA 0.25 mg/L which could guarantee the quality of clustered shoots,was the most suitable hormone matching of clustered shoots inducement.2.Modified KC was the best culture medium in all the medium treatments,and 1/2 MS medium was the second optimum medium.NAA and 6-BA were the suitable auxins and cytokines respectively in vitro culture of Phalaenopsis,and the best multiplication efficiency was obtained in the treatment of NAA 0.5 mg/L+6-BA 7 mg/L.pH 5.4～6 was the best treatment which could promote the explant multiplication of Phalaenopsis.3.All organic compounds could promote the explant multiplication of Phalaenopsis. Leaf juice treatment(5.5 g/L)was the best one in all the organic compount treatments. The concentration of agar adapted to multiplication was 5.5 g/L.Active carbon was the best one in all the treatments,which could control browning and promote explant growth. Except explants preserved at 4℃for 24 hours,the other treatments could decrease browning,but the effects were not as good as active carbon.4.It was important to consider the size of shoots,1 cm which could obtain strong plants,was the best one.The cane sugar could improve the multiplication coefficient significantly,and the suitable concentration was 30 g/L.In practical tissue culture production of Phalaenopsis,the sand sugar could replace the cane sugar as the source on the multiplication growth stage and tap warter could replace distilled warter to reduce the culture cost.5.1/2 modified KC was the best rooting culture medium.IBA was the suitable auxins for rooting culture of Phalaenopsis.All organic compounds could induce rooting of Phalaenopsis,and the best rate and quality of rooting were obtained in the treatment of 70 g/L banana+140 mL/L coconut juice+2 g/L active carbon.Root juice could promote the root of Phalaenopsis grew longer,5 g/L and 15 g/L were suitable concentrations,but it could decrease the number of root of Phalaenopsis.The cane sugar in the medium with suitable concentrations(10 g/L),could significantly promote the quality of rooting.The suitable acclimatization time was 7～10 days.The well-penetrated substance adapted to the plant transplantation in the initial stage and aquatic weed was the most suitable transplantation substance.