| The F1 Seeds of 2 tvarieties of Den.phalaenopsis var. were used as the experiment material, Impact factors, such as component of basal medium, BA, NAA, coconut water (CW) and carbohydrate sources et al., on proliferation and up growth of protocorms for Den.phalaenopsis var. about were studied. The regeneration system for seed-derived protocorms of Den.phalaenopsis var. was also optimized, The F1 seeds of the Den. phalaenopsis var. were used as explants. Most protocorms were induced on 3/4MS -strength Murashige and Skoog medium supplemented with 0.5mg/L BA, 1.0mg/L NAA, 1.0 g/L AC,20.0 g/L white sugar, 5.8g/L agar for 2 mo., The protocorms were mass multiplication on MS basal medium supplemented with BA 2.0mg/L,NAA 0.5 mg/L ,CW 5%, white sugar 20.0 g/L, agar 5.8g/L for 45d,culture 2 mo., plantlets were cultured on 1/2MS basal medium supplemented with IBA 2.0mg/L, NAA 1.5mg/L, CW20%, white sugar 20.0 g/L, agar 5.0g/L for 50d,plantlets growth and development with 4～5 leafs and 3～4 roots. Compared with the reported results , Various elements were decreased to 1/3～1/2 for seed-derived protocorms and plantlet-roots growth in basal medium except for macro- element. But entire regeneration process has shortened 20 days overdue, 5-10 %( v/v) banana juice and potato juice was replaced 20% (v/v) CW that supplemented in medium of protocorms proliferation, not only effect is good , but also usage is more easy .In this present work, RNA was separated from flowers of Petunia hybrida as a pattern plant.A set of primers,which from frame of the origination codon to the ending codon was designed on GenBank's coding AY705977 of ODOI cDNA basis. ODOI gene isolated by RT-PCR,The isolated sequences are 100% matched the sequences in GenBank.The cDNA length of ODOI is 841bp. The expression vector of ODOI was constructed named UBI-pTCK303-ODOI . The UBI-pTCK303-ODOI was transformed to protocorms of Den.phalaenopsis var. by particle bombardment for transgenetic Den.phalaenopsis plant gained.
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