| This test studied effects of berberine (BER) as promoting growth metabolism regulator on growth metabolism, microecology of the caecum and immunity in otter rabbit.First test: Study on berberine improving growth metabolism in otter rabbitsIn this test,40 otter rabbits are divided into 4 groups randomly,10 in each group . The BER of 0, 10, 20, 30 mg dosage were added to one Kg basal diet of otter rabbit. This test was two month. The test studied the effects of different dosage BER on growth, endocrine, the hormone level of serum in otter rabbit. The results showed that:Test groups can improve hormone level of serum.After the 50th day, compared with control group, in the indexes of hormone level of serum, the level of triiodothyronine(T3)increasedrespectively10.13%(P>0.05),17.%(P<0.05),14.7%(P<0.05);thyroxine(T4)increasedrespectively1.56%(P>0.05),22.7%(P<0.05),21.7%(P<0.05);Insulinincreasedrespectively12.7%(P>0.05),82.8%(P<0.01),54.0%(P<0.05);Gluc.reducedrespectively17.6%(P>0.05),24.9(P>0.05),21.1%(P>0.05);CTincreasedrespectively 10.3%(P>0.05),25.1%(P<0.05),21.1%(P>0.05); PTH increased respectively 16.5%(P>0.05),38.3%(P<0.05),12.7%(P>0.05);IL-2increasedrespectively14.0%(P<0.05),21.5%(P<0.01),20.5%(P<0.01);Growth hormone increased respectively 29.4% (P>0.05),115.2%(P<0.05), 115.2%(P<0.05);the level of blood sugar reduced respectively 5.24%(P>0.05),14.6%(P<0.05),12.8%(P<0.05); total cholesterin reduced respectively 38.6%(P<0.05),55.0%(P<0.01),15.0%(P>0.05); total albumen increased respectively 34.0%(P>0.05),37.7%(P<0.05),12.0%(P>0.05); calcium of blood serum reduced respectively 18.7%(P>0.05),31.1%(P<0.05),26.0%(P<0.05); phosphorus of blood serum increased respectively 3.89%(P>0.05),14.3%(P>0.05),8.19%(P>0.05);ammonia-nitrogen increased respectively 0.37%(P>0.05) ,0.12%(P>0.05)and increased 0.25%(P>0.05);SUN increased respectively 9.64%(P<0.05) , 12.4%(P<0.05) , 11.1%(P<0.05);body weight gain increased respectively 25.7%(P>0.05),72.0%(P<0.05),44.4%(P>0.05);average feed intake hay increased respectively 20.4%(P>0.05),39.6%(P<0.05),33.4%(P>0.05).Second test: Study on berberine improving caecum microorganism vitality in otter rabbitsIn this test ,20 otter rabbits are divided into 4 groups randomly,5 in each group . The BER of 0,10 , 20, 30 mg dosage were added to one Kg basal diet of otter rabbit. This test was two month. Caecum fistulas were installed in otter rabbit to research the effect of BER. The results showed that: compared with control group,the pH had no significant difference in test groups;in the dynamic mensuration of animalcule total dehydrogenase activity(TDHA) , test 1 and 2 groups have mighty significant difference(P<0.01) at 12:00,test 3 group have significant difference(P<0.05) at 18:00.In total level, test 1 and 2 groups have significant difference (P<0.05); microbial protein(MCP),test 2 group increased 20.36%(P<0.05) at 6:00,the rest drops have no significant difference;NH3-N level,the average of test groups 3 reduced respectively 1.32%,2.84%,2.44%,there have no significant difference, the orderliness of dynamic change have similarity.Third test: Study on berberine improving immunity faction in otter rabbitsIn this test ,28 otter rabbits are divided into 4 groups randomly,7 in each group . The BER of 0,10 , 20, 30 mg dosage were added to one Kg basal diet of otter rabbit. This test was two month.The test studied the effect of BER on hemaggtulination inhibition antibody titre of Newcastle disease vaccine and the content of lysozyme and bacteriolysin. The results showed that : compared with the control group, after 7 days, special antibody titre of ND of test 2 and 3 grous significantly (P<0.05, P<0.01); after 12 days,test 1and 2 groups have significant difference(P<0.05, P<0.01); after 17 days, test groups have no significant difference(P>0.05); after 22 days, test 1 group has significant difference(P<0.05);the rest drops have no significant difference(P>0.05).The effect of BER on serum-bacteriolysin content has no significant difference.
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