Expression Of LIF, AR And PR In Hypothalamus, Pituitaty Gland, Ovary And Uterus In Gestational Rat

Animal reproduction is a complex physiology process, which could be completed smoothly under the regulation by many factors from inside and outside the organism. The hypothalamic-pituitary-reproductive axis plays a main regulative role and regulates the entire reproduction activity of animal. Pregnancy process of the female animal is complex, for kinds of cytokines and steroid hormones together play an important regulative role in this process. To the great importance, change rule of the leukemia inhibiting factor (LIF), androgen acceptor (AR) and progesterone acceptor (PR) in the pregnancy process may forecast that if the pregnancy succeeds or not. Expression and change rule of LIF, AR and PR in the hypothalamus, pituitary gland, ovary and uterus was studied systematically by using immunohistochemical ultrasensitive SP method in the pregnancy for the first time. We have provided the theory basis in advancing development of early embryo, discovering miscarries as early as possible, enhancing the ration of in vitro fertilization-embryo transfer(IVF-ET) and animal reproductive ability. The main studies results were summarized as follows:1.LIF, AR and PR were discovered in preoptic magnocellular nucleus, medial preoptic nucleus, preoptic periventricular nucleus, optic suprachiasmatic nucleus, supraoptic nucleus, arcuate nucleus, lateral mammillary nucleus. Besides the above nucleus, immunoreactive products of AR and PR were also found in lateral preoptic nucleus, paraventricular nucleus, periventricular hypothalamic nucleus , anterior hypothalamic nucleus, dorsomedial hypothalamic nucleus, ventromedial hypothalamic nucleus, lateral hypothalamic nucleus, posterior hypothalamic, anterior mammillary nucleus, upperior mammillary nucleus, medial mammillary nucleus and posterior mammillary nuleus. Positive products of LIF, AR and PR were located in cytoplasm, cytoplasmic membrane, nuclei and neurite. It showed that LIF, AR and PR played a key role in hypothalamus via genomic and nongenomic mechanism. Strong immunopositive reaction in arcuate nucleus was observed in different period of gestation and that in periventricular hypothalamic nucleus was found in early gestation, which implied that LIF, AR and PR played a vital regulative role during gestational process.2.The immunoreactive products of LIF, AR and PR located in anterior, medialis and posterior pituitary. LIF and AR immunoreactive products mainly existed in the cytoplasm and nuclei, while PR immunoreactive products presented in cytoplasm and nuclei, indicating that LIF, AR and PR participated in the course of physiological function of pituitary. The strongest immunoreaction of LIF, AR and PR was observed in anterior in early gestation. The immunoreaction of AR PR, and LIF was strong, moderate and weak in mid-geststion respectively, and was weak in post-gestation. The results suggested that LIF, AR and PR played a significant role in regulating the reproductive-endocrine activity of pituitary gland in early gestation, especially during the implantation period.3.LIF, AR and PR were widely presented in oocyte, granulose cell, thecal cells, stromal cells, granular lutein cells and germinal epithelial cells. The immunopositive products of LIF was observed in follicular fluid. These three immunopositive products existed in several formation, LIF located mainly in cytoplasm, PR in cytoplasm and nuclei, AR in nuclei of granulose cells, cytoplasm and nucleus of germinal epithelial and in cytoplasm of other cells. The expression of LIF, AR and PR in ovary suggested that they participated in regulation of ovary function. The immunoreactive of LIF, AR and PR was stronger in early gestation than in mid-gestation, and was the strongest during the implantation period, and the weakest in the late-gestation. The findings indicated that LIF, AR and PR played a significant regulative role during the course of embryo implantation.4.LIF, AR and PR could be detected in the luminal epithelial, glandular epithelial, decidual stromal cells, myometrial smooth muscle cells and vascular endothelial cells. The immunopositive products of LIF and AR were located in cytoplasm and nuclei, and that of PR in nuclei. It showed that LIF, AR and PR acted on uterus extensively. The strong immunoreactivity of LIF, AR and PR was observed in all uterine cell types during implantation. The results suggested that LIF, AR and PR could participated in regulation of embryo implantation and development of early embyo.The immunoreactive products of LIF, AR and PR were widely presented in hypothalamus, pituitary gland, ovary and uterus, and level of their expression displayed a regular change rule in different gestation periods. The immunoreaction of them was stronger in early gestation than in mid-gestation and was the strongest during implantation and weakest in late-gestation. The immunoreaction of LIF, AR and PR was strong in arcuate nucleus and anterior of pituitary gland in early gestation, and strongest during the implantation period. Expression of LIF, AR and PR in ovary and uterus has coincidence with in hypothalamus and pituitary gland in implantation. The results indicate that LIF, AR and PR play vital cooperation roles in regulating functions of arcuate nucleus and anterior pituitary gland to ovary and uterus.