Studies On Antifangal Effect And Components Of Essential Oils From Heracleam Moellendorffii

To discover antifungal active compounds from plants is an important way to develop new Reduced-Risk Pesticides. Heracleam moellendorffii is used to medicine belongs to carrot family plants. In this paper, I use different methods to extract essential oil from Heracleam moellendorffii, on the basis of research botanical pesticides in northwest china by Feng Jun-tao,Han Jian-hua etc.The components of essential oil were analyzed by GC/MS.Antifangal active of essential oil extracted by petroleum ether from Heracleam moellendorffii was researched. The results are as follows:1. During the test, three factors based on four levels were considered. The optimum conditions: the size of material(494um), specimen amount(1g), operating mode (after static extraction for 20min, dynamic extraction was made), temperature (40℃), pressure (41.37MPa), CO2 dynamic extraction volume (40mL).The relation of the three factors: temperature>pressure >CO2 volume.2. Essential oil of Heracleam moellendorffii was analyzed and compared with the chemical compounds through three methods: Supercritical carbon dioxide, extracted with petrol ether and hydrostillation from the Heracleam moellendorffii by GC/MS. Its main compounds are: Pimpinellin,á-Sitosterol,1,4,9-Decatriene,1-phenyl-,(E,E)-,Bergaptan ,Methoxsalen,(-)-Spathulenol.3.The Antifungal activity of essential oils from Heracleam moellendorffii extracted by petroleum were tested against 12 pathogenic fungi in vitro by growth rate method .It showed that essential oils from Heracleam moellendorffii appeared to have Antifungal activities to all pathogenic fungi stasis tested in various degrees. Under the conditions texted,it has the strongest inhibitory effect to Rhizoctonia cerealis Vaneler Hoeren(Inhibitory rate is 100%);and the next is Sclerotinia sclerotiorum (Lib)de Bary,Phytophthora capsici Leonian (Inhibitory rate are all more than 80%).