Study On Separation, Purification And Immunological Activities Of Polysaccharides In A Chinese Herbal Medicine-G-Compound Immunoenhancer

Polysaccharides bring new hope for therapying immune-defected disease,tumour,AIDS through the way of non-specific antigen.As compound immunoenhancer,low-toxic and non- rudimental compound polysaccharides will have good market.In this paper, polysaccharides from compound immunoenhancer were extracted and separated and researched the effects on immunity function.The suitable extraction techniques for water-soluble compound polysaccharides from extrusion defatted traditional chinese medical herbs were established.The factors that affected the extraction of compound polysaccharides included the ratio of material to solvent,extracting time, temperature and times of extracting.Aorthogonal experiment was designed .the result showed that the optimal conditions were at the temperature of 60℃, extracting time 4 hour, the ratio of material to solvent1:15 and times of extracting 3.The efficiency of extracting polysaccharides from compound immunoenhancer would be 12.65% at these conditionFifty mice were randomly divided into five groups, Chinese herbal medicine compound polysaccharide group, Chinese medical formula decoction group, astragalus polysaccharide group, normal saline group. A series of immunity tests are carried out. Conclusion: the Chinese herbal medicine compound polysaccharide can obviously increase the immunity function in mice. Chinese medical formula decoction groupis better than astragalus polysaccharide group.During the removing protein process, Sevag method, papain method and Sevage method with papain method together have been compared. The protein scavengin- g rate was 53.7%, 43.9%, 70.8 %; the polysa ccharides losing rate was 35.8%, 3.2%, 12.3 %; The Sevag method with papain method together had not only more polysaccharides extraction percent, but also the highest remov- ing protein percent , so it was theoptimal removing protein process.Three polysaccharides GCPS-1AⅠ, GCPS-2AⅡand GCPS-3AⅢ,were got using ethanol–depositing, DEAE-Cellulose ion-exchange chromatography and TOYOPEARL HW-55F gel chromatography. Three polysaccharides were proved homogeneous by Sephadex G-200 gel chromatography, paper chromatography and ultraviolet spectrophotometer. The relative molecular weights of three homogeneous polysaccharides were determined 398107, 122138, and 7951 by TOYOPEARL HW - 55F gel chromatography.GCPS-2AⅡcan stimulate humoral,cellular and non-specific immunity by oral administration in mice with immunosuppressive agent Cyclophophamide(Cy).JP. injection of Cy can inhibit the proliferation of splennocytes and the genesis of dilated type hypersensitivity,and enhance humoral immunity such as antibody response. GCPS-1AⅠand GCPS-3AⅢcan't reduce immune suppressant caused by Cy.