Polysaccharide Of Extraction And Purification From Traditional Chinese Medicine And Its Effect Of Non-specificity Immune In Mice

Chinese herbal medicine is the Chinese excellent traditional culture's treasure, along with the social and scientific progress and development, the human cognition and constantly updated health concepts,Chinese herbal medicine research at home and abroad, already more and more expected attention. There are polysaccharide, flavonoids, nucleoside, organic acids, alkaloid etc. mainly bioactive substances in Chinese herbals. With the development of molecular biology, and gradually realize the polysaccharide substances in animals, plants and microbes and so on the body cells are found, Constitute the life activities of biological macromolecules essentially(protein,nucleic acid and polysaccharides), one of the basic material, participate in cells of the life activities of adjustment. The researchers found that Chinese herbal medicine of polysaccharides components have regulate immune, antitumor, antiviral, delay ageing, antioxidation, fall hematic fat, fall blood sugar, anti-infection etc. various physiological activity function, one of the immunity activity much researchers attention.This experiment using anhydrous alcohol processing herbal powder, water extraction total polysaccharides composition, and study on the separation and purification technology of Polysaccharide, and researched the effects on immunity function in mice.1,The Chinese Herbal Medicine Compound of crushing degreasing as raw materials. According to the following extraction conditions for extraction:extracting time 24 hours,the ratio of material to solvent 1:10 (Chinese Herbal Medicine Compound:Water) and times of extracting 3. After extraction calculation ratio The Chinese Herbal Medicine Compound total polysaccharide can reach 43.85%. After the ethanol classification, Al, A2, A3 polysaccharide respectively the purity of 49.6%,50.5%,54.7%.2,To AB-8,LSA-5B,DM-18,CAD-40,LX-1,D941 six kinds of macroporous adsorption resin for material, through the static and dynamic adsorption and desorption experiment, we studied the above 6 kinds of resin adsorption and desorption performance of compound polysaccharide. Results show that:AB-8 resin is preferable candidate for separating compound polysaccharide.3,Though single-factor and orthogonal experiment design to determine the optimal parameter adsorbent and desorption of AB-8 for compound polysaccharide. The best conditions is:5.93 mg/mL polysaccharide concentration, rate 3BV/h,amount of polysaccharide 1BV,adsorption ratio is74.3%. the elution agent for the 50% ethanol, elution rate 3BV/h.eluting dosage3BV desorption at best, desorption rate is 93.6%. A1, A2, A3 via macroporous resin purification, income B1, B2, B3 polysaccharide respectively the purity of 79.8%.77.1%,64.7%.4,To observe the effects of polysaccharides from the Chinese Herbal Medicine Immunoenhancer on immune functions in immunity mice. Mice irrigation respectively take medicaments (compound total polysaccharide, compound water decoction, three different components, high purity three different components, physiological saline), detection of red blood cell count, leukocyte count, measure leukocytes classification number, lymphocyte proliferation rate, macrophage phagocytosis rate, EA wreath rate, ANAE dyeing, immune organs index.Synthetize these results, high purity components two had the best effect on immune function than all the other medicines.