| A molecular genetic map for Chinese cabbage was constructed based on AFLP markersusing DNA samples extracted from 172 F2 populations obtained through A52-2 and GC4805cross. QTLs analysis of Chinese cabbage resistance to TuMV-C3 was carries out. The mainresults are as follows:1. By ELISA detection and phenotypic trait observed under the condition of artificialinoculation at seedling stage in greenhouse, we initiatived that the resistance of Chinese cabbagetoTuMV-C3 was controlled by incomplete dominant genes, assumed qualitative genetic characterand additive effect, so F2 population could be used to set up the genetic linkage map and locateQuantitative trait.2. Using parents and F2 population to screen 64 paires of primers, we gained 273 AFLPmarkers. By the analysis of 273 AFLP markers with Mapmaker/EXP3.0b software, weconstructed molecular genetic map for Chinese cabbage, a total of 156 AFLP markers weremapped to 10 linkage groups, covering 1090.3 cM with an average distance of 7.0cM. Thenumber of markers in every linkage group varied from 8 to 23, the total map distance from 36.7to 168.6cM.3. 5 QTLs controlling TuMV-C3 resistance were identified with Windows QTLCartographer V2.0 software and Interval Mapping method. 5QTLs named R01,R02,R03,R04,and R05,were separately located on linkage group LG1,LG1,LG3,LG4,LG4 and theexplainable variation were 13.68%,13.49%,9.03%,13.06%,11.13%. So we concluded that theresistance of Chinese cabbage to TuMV-C3 was controlled by at least five pairs of dominantgenes and the dominance was incomplete. QTL analysis results could be applied inmarker-assisted selecting (MAS) for Chinese cabbage breeding. And it also provided theoreticalbase for improvement of Turnip mosaic virus (TuMV) resistance in Chinese cabbage.
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