Hot Pepper Correlation Analysis Among Physiology Biochemistry Characteristics, Agronomic Characters And Resistance To Disease Characters

12 kinds of hot pepper materials were used for experiment. The correlation amongphysiological and biochemical characteristics, agronomic characters and resistance todisease characters was studied and the genetic relationship of 12 pepper materials wasidentified by using POD isozymes, the content of capsicin was determined by HighPerformance Liquid Chromatography. The results were reported as follows:(1) By simple correlation analysis, fruit weight was positively correlated with fruitlength, fruit width, fruit petiole length and flesh thick, but it was negatively correlatedwith fruit number. Plant height was most significantly positively correlated with plantspread. Fruit length was most significantly positively correlated with fruit petiolelength, and significantly correlated with fruit weigh. Fruit width was significantlypositive correlated with flesh thick, and most significantly positively correlated withfruit weigh, and most significantly negatively correlated with fruit number. Capsicumcontent and fruit flesh thick were most significantly positively correlated with fruitweigh and PPO activity, and significantly negatively correlated with fruit number.Dry matter content of fruit was significantly correlated with capsicum content of fruit,and most significantly positively correlated with CMV virus disease index. Capsicumcontent of fruit was significantly positively correlated with CMV virus disease index.POD activity and PPO activity were significantly positively correlated with epidemicdisease index.(2) The canonical correlation coefficients were significant between production factorcomponents (PFC) and fruit character (FYC), resistance to disease (RDC)andphysiological and biochemical index (LPI), resistance to disease (RDC)and fruitnutrition content(FNC). The canonical correlation coefficients were not significantbetween others. In group which had significantly correlation to multiple charactergroups, the largest canonical correlation coefficient contained smaller ratio of geneticcorrelation information in total genetic correlation information than other singlecharacter group.(3)Canonical correlation analysis showed that fruit weight was significantly positively correlated with fruit length, fruit width, fruit petiole length and fleshthick..Resistance disease characters were significantly positively correlated withPOD activity, PPO activity, Dry matter content of fruit. Resistance disease characterswere negatively correlated with PAL activity, but was not significant.(4) Isolation effectiveness of gel with different concentration: 10ï¼…concentration ofgel was suited for both of POD and PPO isozyme analysis. Clear enzyme band washelpful to do correlative analysis. Genetic distance of 12 varieties changed from 0 to0.51.They could be divided into three kinds of species when Genetic distance was0.36, six kinds when 1.9(5) Chromatography factors of Determination of capsicin by high performance liquidchromatography: The mobile phase was V(CH₃OH): V(H₂O)=75:25 with a flowrate of 1.2ml/min, UV detector set at 280nm, columniation temperature 25℃. Thecapsicin contents of 12 hot peppers were 0.07 to 8.02 mg/g. The hottest wasWild-hill-pepper-1 in Yaan, which contained the highest capsicin. In the next placesof capsicin contents were Wild-hill-pepper-2, To-sky-pepper-2 andTo-sky-pepper-1.The Jiaozhou-long-pepper from Jiaozhou in Shandong provincecontained lowest capsicin.