Analysis Of Medicago Sativa L.,M. Varia Martyn. And M. Falcata L. With SSR Molecular Markers

With SSR molecular marker technology, the genetic variability of 150 individuals of 6 alfalfa populations was analyzed in this thesis. The materials consisted of 1 M. Sativa L.,2 M. varia Martyn and 3 M. falcata L.The results shows that:(1) Established SSR-PCR amplified condtion on Medicago facalta L.: in total volume of 25μL PCR reaction,0.2mmol/L dNTPs,2.0mmol/L MgCl2, 80ng templ-ate DNA,0.16μmol/L primers and 1U TaqDNA polymerase were the best consist-ence.(2) The 4 SSR loci with ideal bands were slceted from 10 pairs of SSR pri-mers. 59 alleles were found in them.The allele frequencies were calculated with 0/1 method.(3) With the allele frequencies, we calculated the genetic heterozygosity, pol-ymorphism information content (PIC), and effective allele number. A high level ofvariability within populations was found in 6 alfalfa populations. It was shown th-at the PIC of all loci were more than 0.5. Among these 4 loci, The highest PIC was 0.9196 in MAA660456. the range of average genetic heterozygosity of each population was from 0.8230 to 0.8765, and that of average effective alleles numb-er of each population was from 4.95 to 12.83.(4) With the MVSP_V3.1 software, genetic resemble coefficient and genetic distance (Ds) among populations were calculated and the UPGMA dendrogram wasconstructed to evaluate the genetic variability and genetic relationship among populations. These 6 populations could be regarded as three cluster. Number 2,3,4,5 in the 6 populations were regarded as one cluster, either of number 1 and number 6 population could be regarded as one cluster.