| Field experiments in Longyan city Fujian province and Yiyang county were conducted to study the effect of plant growth regulators on grow development, interior chemical components, the neutrality volatile aroma and yield and value at the upper leaves of flue-cured tobacco (K326) from 2006. The results as follows:1. After praying plant growth regulators, it had a great effect on increasing single leaf areas and reaching significantly different compared with CK. According to the single leaf areas, the better treatments are A3, B3 and C2.2. The decreasing speed of chlorophyll and carotenoids by spraying plant growth regulators after topping was slowed. The changing trends of chlorophyll and carotenoids by spraying different concentration BR and MJ were decreasing-increasing-decreasing,compared with decreasing continuously by spraying different concentration GSH, mixed reagents A and B.As the concentration of BR and MJ decreasing and the concentration of GSH increasing, the slowing effects on chlorophyll were reinforced; Meanwhile as the concentration of BR decreasing and the concentration of MJ and GSH increasing, the slowing effects on carotenoids were reinforced, the slowing effects on chlorophyll and carotenoids by spraying mixed reagents A were higher than that of spraying mixed reagents B. The treatments of higher content of chlorophyll was A3 , B3 and C1 is; The treatments of higher content of carotenoids was A3,B1 and C1 ;The treatment of the slowest content of chlorophyll and carotenoids was E.3. The changing trend of amylase of treatments spraying exogenous plant growth regulators after topping was basically consistent. The trend of amylase was decreasing after the second spraying then increasing. As the concentration of BR and MJ decreasing, the activities of amylase were increased, and spraying middle concentration of GSH had the highest amylase activities and spraying mixed reagents A had higher amylase activity than mixed reagents B. The treatments of higher amylase activity were A3, B3 and C2. After topping invertase activities decreased and invertase activities by spraying exogenous plant growth regulators slowed. As the concentration of BR and MJ decreasing, the slowing effect was strengthened, and the slowing effect of the middle concentration of GSH was strengthened, then mixed reagents A had higher slowing effect than mixed reagents B. The treatments of higher invertase activities were A3,B3,C2 and D.After topping PAL activities decreased. The treatments of higher PAL activities were A1,B1,C1 and D.After topping SOD activities decreased and SOD activities by spraying exogenous plant growth regulators slowed. As the concentration of BR and MJ decreasing, the slowing effect was strengthened. The treatments of higher SOD activities were A3,B3,C1 and D.The changing trend of MDA content after topping was decreasing -increasing. The MDA content by spraying BR was slower than that of CK, as the concentration of BR increasing, The MDA content was increased. With the concentration of MJ increasing and GSH decreasing, The MDA content was increased. Spraying mixed reagents A had higher MDA content than Spraying mixed reagents B. The treatments of slower MDA content were A3,B3 and C1.After topping soluble protein content decreased. Spraying BR and high and middle concentration of MJ increased soluble protein content and other treatments decreased soluble protein content, meanwhile with the concentration of GSH decreasing, soluble protein content was decreased. The decreasing magnitude of soluble protein content mixed reagents A was higher than that of mixed reagents B. The treatments of slower soluble protein content were B1,C3 and D.4. The changing trend of starch content was increasing-decreasing- increasing and all treatments reduced starch content at the upper leaves of flue-cured tobacco. With the concentration of MJ and GSH increasing, the degradation content of starch increased. The treatments of slower starch content were A1,B1,C1 and D.The changing trend of total sugar content was decreasing -increasing-decreasing as a whole and every treatment increased total sugar content at the upper leaves of flue-cured tobacco. With the concentration of BR,MJ and GSH decreasing, the content of total sugar increased. Spraying mixed reagents A had higher total sugar content than Spraying mixed reagents B. The treatments of higher total sugar content were A3, B3, C1 and D. The changing trend of reducing sugar content was decreasing -increasing-decreasing in general and other treatments except B1 increased reducing sugar content. With the concentration of BR and MJ decreasing, the content of reducing sugar increased. The magnitude of increasing reducing sugar content of middle concentration of GSH was highest. Spraying mixed reagents A had higher reducing sugar content than Spraying mixed reagents B. The treatments of higher reducing sugar content were A3,B3,C2 and D.5. After topping nitrogen content decreased. Spraying different concentration of BR and MJ increased nitrogen content and with the concentration of BR decreasing and MJ increasing, nitrogen content increased; Different concentration of GSH,mixed reagents A and B decreased nitrogen content, the magnitude of decreasing nitrogen content of middle concentration of GSH was higher. The treatments of slower nitrogen content were A1,B3,C2 and D.After topping nicotine content of spraying MJ ,mixed reagents A and B increased; The changing trend of nicotine content of spraying BR and GSH was increasing-decreasing- increasing and the peak was second spraying. with the concentration of MJ decreasing , nicotine content decreased and high concentration BR,middle concentration GSH and mixed reagents B decreased nicotine content. The treatments of slower nicotine content were A1,B3,C2 and D.The changing trend of free Amino Acid was decreasing- increasing- decreasing and the peak was third spraying. Other treatments except D and E increased free Amino Acid content. With the concentration of BR decreasing and MJ increasing, free Amino Acid content increased. The free Amino Acid content of spraying high and slow concentration of GSH was higher than that of middle concentration of GSH; spraying mixed reagents B had higher free Amino Acid content than that of spraying mixed reagents A. The treatments of slower free Amino Acid content were A1,B3,C2 and D.6.Other treatments except B3 had slower total sugar content than that of CK and reached remarkable discrepancy compared with CK; Other treatments except B2 and B3 had slower reducing sugar content than that of CK; Other treatments except A2 and A3 had slower nitrogen content than that of CK; Other treatments except A2 ,A3 and E had slower nicotine content than that of CK;All treatments had slower starch,potassium and chlorine content than that of CK. Other treatments had slower ratio of potassium and chlorine except A3 and D than that of CK and the treatments of A2,B1 and B3 reached remarkable discrepancy compared with CK; All treatments of ratio of total sugar and nicotine reached remarkable discrepancy compared with CK. The treatments of coordinated interior chemical compositions were A3,B3,C2 and D.Other treatments except B3 had higher aromatic components total content than that of CK; All treatments had slower carotenoids degradation content and neophytadiene content than that of CK; Other treatments except C1 and C3 had higher cembratriendid compounds content than that of CK; All treatments had higher hypoglycemia compounds content and browning reaction content than that of CK.Other treatments except B1,C3 and E had higher yield than that of CK; Other treatments except B1,B2 and C3 had slower proportion of high grade leaves than that of CK; All treatments had higher proportion of middle grade leaves than that of CK; All treatments had higher value than that of CK; Other treatments except A1 had higher average leaf prices than that of CK; The treatments of were higher comprehensive economic benefit A3,B3,C2 and D. |
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