Construction Of RIL Population And Preliminary Study Of Genetic Map

Rice is one of the major crop for human consumption, providing staple food formore than half of world's population. Most important agronomic traits of rice arequantitative, such as yield, quality and stress resistance and so on, which are controlledby polygene, so It's very important for rice breeding improvement to map quantitativetrait loci (QTLs) accurately. Specially, it is very useful to clone QTL using thematerials which genomic full-sequence has been know. in this study, using thecombination of Pei'ai64S/Nipponbare for constructing RIL population, evaluated RILpopulation based on phenotype value and genotype value, we constructed rice SSRlinkage map based on RIL population, compared it with the F₂ map from the samecross Pei'ai64S/Nipponbare and the sequence map of Nipponbare, respectively. weanalyzed the segregation markers, then 3 types of agronomic QTL were mapped on themap. The main results were as the following:1.Using the F₁ descendants of combination Pei'ai64s/Nipponbare for constructingRIL population, F₂,F₄,F₆,F₈ were planted in wen jiang county Sichuan provinceand F₁,F₃,F₅ F₇were planted in lingshui county hainan province, respectively. Thepurity degree of 3 types traits (tiller number,panicle number,plant height) is60.61%,90.0%,50.61%, respectively. The purity degree of RILpopulation withmolecular marker evaluation is 96.03%,mixed degree is 3.97%, respectively.2.Examined polymorphisms of biparent by 515 primers, there were157 primersShowed polymorphisms, the ration of polymorphisms was 30.48%, we used theseMarkers for constructing a linkage map of 109 markers, including 18 linkage groups,the total genetic distance of the map was 2732.6cM, the average distance of twoadjacent markers was25.07cM.3.compared with F₂map, there were great difference about linkage,number ofmarkers,marker order,total genomic length,average marker distance on two maps;compared with G-map, of the 109 markers, 91 were tagged to G-map, others 18markers were not located on the same chromosomes on G-map, 74 SSR markers,covering 159.8 Mb of genomic length with an average interval size of F₂map2159.46kb.4. There were many significant segregation distortion markers in RIL population,63 markers (68.48%) were distorted on the F6map, (P＜0.05), of 63 markers, 60markers were deviated toward Pei'ai64S, only 3 markers were deviated towardNipponbare; there were others 65 markers without mapping, 27 markers weredistorted,38 marker were segregated normally.5.60markers (95.24%) showed deviation on the map were mappedchromosomes1,2,3,4,5,6,8,9,11,12, respectively, most markers werein segregation distortion regions, named as followed: SDR1-1,SDR1-2,SDR2,SDR3-1,SDR3-2,SDR4,SDR5-1,SDR5-2,SDR6-1,SDR6-2,SDR8,SDR9-1,SDR9-2,SDR11å’ŒSDR12. Most of SDR deviated toward Pei'ai64S, only two markersof SDR1-1 deviated toward Nipponbare,...