QTL Analysis Of Some Agronomic Traits In Qing-Tibetan Plateau Annual Wild Barley

Barley is one of the most oldest cereal crop in the world, mostly used in feed, grain,and industrial material for beer, medicine as well as health protection grocery, andoccupies the fourth place which is only inferior to the place of wheat, rice and maize.Barley is also model plant for genetic researches. Recently, with the development of beerindustry and the health function and nutritive of barley is attached more and more, theresearch of the barley breeding reaches a new stage. The Wild Barleys from Qing-Tibetanplateau which have a large number good gene has great potential in barley breedingresearch and attracted many people's attention.In this paper, the F2 population which constructed for genetic mapping were madefrom the hybrid combination crossed by a Qing-Tibetan plateau annual wild barley and acultivated barley in Australia. Construction of molecular genetic map of barley by SSRtechnology, after then analysis and location of genes controlling dormancy and otheragronomic traits. Conclusion of this experiment are as following:Ⅰ. Investigation and analysis of the 11 agronomic traits which are plant height,spike number per plant, main spike length, uppermost inter-node length, distance of flagleaf phyllula to neck, awn length, spikelet number of main spike, grain number of mainspike, grain number per plant, grain weight per plant and 1000 grain weight of twoparents and F1, the distance of flag leaf phyllula to neck, awn length, spikelet number ofmain spike and 1000 grain weight are significant difference at 0.05 level. According to theresult of awn length and 1000 grain weight have significant difference at 0.05 levelbetween reciprocal crosses F1, that can suppose these two traits maybe inheritedmaternally or with maternal effect.Ⅱ. The correlation analysis of 11 traits and the normal probability distribution areindicated: the 11 traits have good correlation, and the correlation coefficient is higher than0.800 between spike number per plant and grain number per plant, grain weight per plant;uppermost inter—node length and distance of flag leaf phyllula to neck; main spikelength and spikelet number of main spike; grain number per plant and grain weight perplant in F2 population. The 11 traits without grain number of main spike are all normalprobability distribution and suitable for QTL location.Ⅲ. 49 pairs of SSR primers were used for polymorphic analysis between the two parents and construct a molecular linkage map. This map consists of 7 linkage groups,covering 290.3cM with an average distance of 8.3 cM, the shortest of 0.1 cM and thelargest of 38.6 cM between locus.Ⅳ. Based on the newly constructed genetic map of barley, QTL mapping andanalyzing of the 11 traits, with composite interval mapping (CIM) method. The resultsshowed that a total of 6 QTL controlling 5 traits were detected, and controlling spikenumber per plant is two, the others each one. 4 QTL controlling 4 traits which arecontrolling distance of flag leaf phyllula to neck, spike number per plant, grain numberper plant and grain weight per plant each one is located on the seventh groups in thesimilar region by clustering distribution. The rest QTL of controlling grain number ofmain spike and another controlling spike number per plant is located on the forth groupand the sixth group respectively. The phenomenon of 4 QTL in the similar region byclustering distribution shows that maybe they are the same QTL or proves the correlationanalysis results, and match to the phenotypes conditioned by multiple micro-effect genes.Ⅴ. The maximum contribution in this 6 QTL is qSFL-07-22.69 controlling distanceof flag leaf phyllula to neck can explain 7.55% phenotypic variance linked to the SSRmarker BMAG767 and EBMAC827and EBMAG757, showed additive effect anddominant effect from the parent Hua 0572 and Homelin respectively. The minimum isqSNP-07-16.59 controlling spike number per plant can explain 4.08% phenotypicvariance linked to the SSR marker BMAG11 and AWBMS37, showed additive effect anddominant effect from the parent Hua 0572 and Homelin respectively as same asqSFL-07-22.69 the. From the information above, our studying will be helpful to themolecular assistant breeding.