Studies On Hubei Magnaporthe Grisea Species Identification And Evaluation Of Rice Blast Resistance

Rice blast(Magnaporthe grisea(Hebert) Barr(the anamorph is Pyricularia grisea (Cooke) Sacc.) is one of the most important rice disease that widely occurred in all rice belts in the world. At present, the control of the disease to take planting resistant varieties mainly of integrated management measures. But the pathogenicity are genetic variability and diversity often leads to the loss of new rice varieties resistant, The study pathotypes sexual differentiation, disease-resistant varieties of breeding, and forecast the prevalence of disease is extremely important. Long practice shows, breeding, planting resistant varieties of rice blast is the most cost-effective preventive measures. Research, analysis of blast resistance biochemical mechanism for rice blast resistance lasting efficient use of chemical control of the new drug development, resistance gene cloning and breeding resistant varieties with the theoretical and practical significance.The test consists of three parts : different regions Blast Races Distribution; different varieties of rice blast resistance emotional inoculated with identification; rice varieties resistant to flu Blast physiological and biochemical mechanism. Race application of a unified national seven varieties of rice in Hubei Magnaporthe grisea species identification. The results showed that from Hubei 16 counties (cities) in 15 varieties of rice were obtained 56 isolates can be divided into six groups of 28 races, most of the Indica small stocks, which ZA dominant population, fragments dominant races. According to the analysis, Hubei rice Magnaporthe grisea species has become increasingly complex dynamic, and in different regions and the distribution of species differences are more obvious. Huanggang physiological various areas spread more, ZA, ZB, ZC and four Stocks ZG 13 races, the fact that many of the Indica small stocks.Identification of resistance using Blast advantage Hubei Races ZA strains of Hubei collected throughout the 91 major water Rice varieties of artificial inoculation blast identification. Participation of 91 Stephen Rice, the blast has not yet been found highly resistant varieties. Anti-resistant varieties or share of less than 25%, and the rest are susceptible varieties or the flu. Only 2.2% of varieties for resistance.Screening by anti-flu varieties of rice blast, emotional confrontation significantly different varieties for the physiological and biochemical mechanism. Screening study on the four varieties defense activity and the content of soluble proteins, found peroxidase (POD), phenylalanine ammonia-lyase (PAL), Superoxide dismutase (SOD) activity of three defensive changes and the resistance of rice a certain emotional relevance. POD is a rice blast resistance one of the important mechanisms, and its activity and disease resistance are positively correlated. POD isozyme course early activity is also conducive to rice blast resistance. SOD activity in the early changes, rice blast resistance one of the important mechanisms, blast resistance and its activity was negatively correlated. PAL activity level of rice blast resistance is an important mechanism, PAL activity levels and peak moming and evening arrival time of blast resistance and close contact with PAL activity, peak arrival in the long-blast to play. Soluble protein and rice blast resistance there is a positive correlation between. Soluble protein content increased speed, rise time earlier, the growth rate is conducive to blast resistance expression.