Applied Studies On Selection Of High Quality And High Quantity Parameters Of Pleurots Serotinus Strains

Hohenbuehelia serotina(Perts: Fr.) Sing. is a famous native product which has flesh, good smell and plenty of nutrition. Pleurots serotinus has cultivated successfully in China but not reported on the changes of cultural characters and enzyme activity during the growth of mycelium and the selection of Pleurots serotinus strains. In this paper, Improvement system chosen method(multispore hybridization)was used to breeding from Hohenbuehelia serotina on the base of results in others edible fungus; study of nutrition and growth conditions by pure culture;Different culture mediums were investigated to choose the best, aiming to research the change of activities of the enzyme and difference of quantity and quality of mature fruitbodies. The results are as follows:(1) 100 combinations were formulas by multispore hybridization. 19 were formed catenation. Received four hybridization strains which had different inheritable characters according to physiological characteristic and results of esterase isoenzyme.As a tesult,filtering a best strain"HE-5"which had better growth potential and rate of growth.(2) The optimum temperature is 25℃and reach the maximum of 4.77cm, next is 20℃, Pleurots serotinus grows slowly under the condition of 30℃and get the minimum of 2.626cm. The most suitable pH is 6.5 and reaching the most value of 5.68cm, The worst growth is at>pH7.5.and3,NH₄NO₃,KNO₃ and bran. In the study of C/N, 35:1 was greater than 10:1 and 40:1. In the experiment of mineral salt, MgSO₄ was the best greater than K₂SO₄ and ZnSO4,the growth of mycelium was 5.76cm.(4)The optimum temperature, carbon, nitrogen and mineral salt chose through the test of environment and nutrition condition were used to make L9(34) orthogonal experiment. The results showed that the suitable culture medium parameters are: 25℃, 5g beef extract, 20g maltose, 21gCaCl, H₂O1L.(5)The research on the mycelium growth of No. 5 strain of the extracellular enzyme activities on different culture mediums showed that different chemicals could influence the activities of extracellular enzymes(cellulase, hemicellulase, ligninases, amylase). The change trend of CMC enzyme is raising before the fruitbody maturing and reaching the maximum then falling gradually; hemicellulase and amylase were reverse to the CMC enzyme, E formula has biggest change and C was smallest; The content of ligninases and laccase was always high during the mycelium growth and then descends after the anlage formed.(6)The culture mediums of saw dust: 50% wheat bran, 15% corn cob30% and 5% plaster is the best parameters in this study. Its fruiting body is 8.9cm,yellow,and abnormality shortly and better selling.Its efficiency of biology is 105%.So, In this paper we breeding a better strain of Hohenbuehelia serotina, making definite the culture condition and enzyme activity, proposing the best and optimum formula.