| In this paper research the effect of microorganism in the formation and structure without organicmaterials,and divided into indigenous microbes and microbial alien microbes two categories, They studiedthe soil humus photochemical nature and the effects of the structure.In order to reveal differentmicroorganisms in the soil humus into the role and structure of humus in theory;in practice, the study ofsoil reclamation of farmland soil humus decline in the mechanism. Through experiments to understand theamount changes of organic matter, Humus composition and chemical properties and optical properties withtime, clearly different microbial plays an important role in the formation of the organic matter. Providing atheoretical basis to illustrate the microbiology of humus formation and changes in the structure of the roleplayed by. Experimental studies show:(1)The change of microbial and organic carbon show a downward trend over time. The quantitydecreased are consistent with the changes of soil organic matter, soil carbon content affect the amount ofmicrobes directly.(2) Extraction of humic substances HE present first increase and then decreasing slowly trend, In earlymicrobial HE promoted the formation, HE started to decline with the extension, HE fungi on thedecomposition rate greater than the rate of formation.(3)The relative contents of HA are in the increase trend,it shows that the selected strains acceleratingthe formation of the HA. The relative contents of FA slow increase first then declining trend.(4)In the early culture,the PQ of indigenous bacteria and alien take on first decline then increasing,Show that the initial formation of FA hyper then HA, with time FA change into HA, PQ increased. The PQof indigenous fungi are in a steady decrease fron 1 to 120 days, after the PQ changes become calm. Itpresent that the formation speed of HA, FA to a balance; 180d stage after PQ increasing, HA changes to theFA.(5)In the entire incubation period, the HA processing LogK previous 90 days showed a significantdownward trend. At the beginning of culture, LogK are higher, indicating that the initial training HArelatively simple molecular structure. With the extended incubation time, LogK decrease, they show themolecular structure to the complex gradually in the direction of development. Until 90 days after the end oftraining, LogK value has been in a steady state, HA molecular structure tends to be stable. The processingof LogK is in little difference. (6)The LogK of the FA has increased in the previous 30 days, indigenous bacteria in the next 90 daysmarked a downward trend show that FA began to creating high humus of the components, molecularstructure to the complex gradually in the direction of development, after a small increase before stabilizing.The LogK of external bacteria has been in a previous downward trend and the late is basically the samewith indigenous bacteria. The LogK of Alien fungus value has been exhibited at the previous downwardtrend and the late foreign bacteria trend is basically the same. It was also, the FA's value is greater than theLogK of HA, it shows that the molecular structure FA was simple than HA, and the degree of oxidation ofaromatic was low.(7)Indigenous bacteria and fungi increased foreign HA aliphatic C content, improving the aliphaticHA, foreign indigenous bacteria than fungi significantly; indigenous fungi and bacteria outside an increaseof the aromatic C HA.(8)The IR waveform basically the same, but the chemical shifts of the absorption peak intensitydifference. Note that HA has the similar structure of C frame. In different culture period in addition to themolecular structure which is common, indigenous bacteria and alien fungus has not extend phthalic amineC=O, indigenous fungi amine C=O stretching Phthalocyanine (1602.59) decline, alien bacteria has phthalicamine C=O stretching, Note that alien bacteria may s play an important role on the humus nitrogen source.In addition, there were N-H swinging in the amino acids after training 180 days. |
