Establishment Of Regeneration System Of Mature Embryo And Anatomical Research Of Elytrigia

The study in this paper using mature embryos of E.elongata,E.intermedia and Elytrigia hybrid (E.elongata X E.intermedia) as explant , cultivate them in different culture medium,way of inoculate and processing time of 4℃to find how to effect the growth of callus, and to find the correlation between genotypes, hormone ,AC and callus differentiation . The results were as follows:(1) The best way of inoculate mature embryos was: placed embryo upwards and made the scutellum tounched medium completely. This way was propitious to the frequency of callus induction.(2) Treating seeds at low temperature 4℃had certain effect on callus induction, the effect depended on varieties and treatment time. The treating for 5days was suitable for E.elongata,E.intermedia, and their callus frequency reached 98.51% and 89.66% respectively.(3) The feasible medium of E.elongataThe callus inducing medium: MS+CH(500mg/L)+2,4-D(3.0mg/L)+6-BA(0.1 mg/L);Subculture medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+6-BA(0.5mg/L); Differentiation medium: MS+CH(500mg/L)+6-BA(4.0mg/L)+NAA (0.5mg/L).(4) The feasible medium of E.intermediaThe callus inducing medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+6-BA(0.1 mg/L);Subculture medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+6-BA(0.2 mg/L); Differentiation medium: MS+CH(500mg/L)+6-BA(3.0mg/L)+NAA (0.5mg/L).(5) The feasible medium of Elytrigia hybrid (E.elongata X E.intermedia) The callus inducing medium: MS+CH(500mg/L)+2,4-D(1.0mg/L)+6-BA(0.2 mg/L);Subculture medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+6-BA(0.2mg/L ); Differentiation medium: MS+CH(500mg/L)6-BA(4.0mg/L)+NAA (0.5mg/L) +AC(4g/L).The other study in this paper is about anatomic structure analysis of roots and leaves of Elytrigia which have nearly same structure but different character. For example, in the roots of Elytrigia hybrid (E.elongata X E.intermedia) there are more passage cells, and in the leaves, the cuticles are thicker.