The Cloning And Sequence Analysis Of The CDNA Of Cow Bone Morphogenetic Protein Receptor-IB Gene

Bone Morphogenetic protein Receptor-IB Gene (BMPR-IB) affecting reproduction performance of cow has been researched. Using the sequence information of other species in the Genbank, through megalign, analysed it`s sequence and method of RT-PCR, we magnified cloning and the sequence analysis of the cow BMPR-IB that didn′t report yet. The method was: first, searching for the BMPR-IB gene sequence of human, rat and sheep in the Genbank, we analysed the homology of the BMPR-IB gene sequence by using DNAStar software. According to the homology of the longer cDNA sequence we designed the primer. We used the TRIzol Reagent to extract the total RNA from the cow ovary. adopted RT-PCR technique to magnify the parts of cDNA sequence of the cow BMPR-IB with it as template, and reorganized this fragment to the PMD18-T vector, slided it by two different enzymes and PCR identification, we acquired a part of cDNA sequences of the cow BMPR-IB for 953 bps and the cloned fragment codes 389 amino acids. 2,Comparison of the gene sequences with goat,sheep,human,monkey,chimpanzee,house mouse,Norway rat,pig,opossum,dog and chicken from Genebank , The nucleotide homology were 98%,97.6%,91.9%,91.7%,92.1%,87.9%,87.3%,92.9%,86.8%,91.6% and 83.9% respectively,We got a result that this cloned sequence is the BMPR-IB sequence of cow. It is important basis for cloning other BMPs family.3,At the same time, we analysed the amino acid sequences. The homology in 11 kind of animals mentioned which with the surfaceis were all above 98%.Protein Secondary Structure Prediction of BMPR-IB gene offer help for understanding cow reproduction mechanism. This sequence has been registed in the Genbank, and the number is DQ489533.