The CDNA Cloning Of BMP-4,BMPR-IB And The Expression Of BMPR-IB Gene In Inner Mongolian Cashmere Goat

The cashmere comes from the second hair follicle of Cashmere goat. It is very important to study the mechanism of molecular regulation about skin follicle development, and it has a direct influence to the quantity and quality of cashmere .This experiment regards the Inner Mongolia Cashmere goats as sdudy materials.The BMP-4 and BMPR-IB cDNAs were cloned from gaot skin tissues. we acquired a part of exon sequences of the gaot BMP-4 and BMPR-IB for 306 bps and 606 bps. Sequence analysis about BMP-4 and BMPR-IB indicated that there were high homologies of nucleotide acid between goat and human or other mammalian.Tissue specific expression of the BMP-4 and BMPR-IB gene was also investigated in cashmere goat by RT-PCR analysis. The results showed that BMP-4 and BMPR-IB gene was expressed in different period of the tested skin tissues, including embryonic stage, anage, telegen and catage. So we can presume that BMP-4 and BMPR-IB gene could perform multiple functions in the regulation of skin appendage morphogenesis and the postnatal growth of hair follicles.In situ hybridization study showed that mRNA of BMPR-IB gene was expressed in epithelial cell, hair matrix, dermal papilla, inner root sheath,outer root sheath and hair shaft during the hair follicle development. Meanwhile. This result showed that BMPR-IB gene might play a role for regulating hair follicle morphogenesis, development, differentiate, programmed cell death and cyclic change.