| There are a lot of genes which relates the reproductive traits of sheep, among them may be have a major gene, and also the major gene which control the multiple births may be different in different sheep breed or strain. Therefore this research has received increased interest in recent years.In this study the polymorphisims of BMPR-1B、BMPR-IA、BMP、GnRHR、PGR、INHA、FSHβ and PTGS2genes in Duolang sheep (Prolific population-PP)-Duolang sheep (General population-GP), Karkol sheep (KS) populations were analyzed by the technique of Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP), Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR-RFLP) and Gene sequencing. At the same time, attempt to find the link of high productivity of Duolang sheep (Prolific population) with those candidate genes. The results were as follows:(1) The polymorphism of BMPR-1B gene in Sheep was detection by PCR-RFLP method, the A746G mutation in BMPR-IB gene was found only in PP. The A allele of the A746G mutation was the dominant gene, the average litter size of BB genotype was20.91and0.9greater than those with genotype AA and AB.(2) In Duolang sheep, two genotypes of BMPR-IA gene were found (AA and AB); the genotype frequency was:0.42and0.58respectively, no significant difference (P>0.05). The sequencing results revealed that six single nucleotide mutations were found which did cause5amino acid changes. The other sheep breeds or strains are AA genotype.(3) Exon3of BMP4gene had three genotypes (AA, AB and BB) in PP, the genotype frequency were0.26,0.64and0.1respectively, AB>AA>BB, there was no significant difference between them (P>0.05). Two genotypes were found in GP, which are AA and AB; the genotype frequency are0.85and0.15, no significant difference (P>0.05). Two genotypes were found in KS, which are AA and BB; the genotype frequency are0.03and0.97, no significant difference (P>0.05).(4) When there was no report about the polymorphism GnRHR gene in Xinjiang Duolang sheep, polymorphism of of GnRHR gene were detected. Three genotypes were found only in PP, the other sheep breeds or strains has AA genotype; For PP, the BB genotype had0.65and0.76(P<0.05). The sequencing results revealed that four mutations were found, respectively, A'T, A'T and A'*, at No.50,54and79bp in this gene were found.50A'T and54A'Tleaded Lys, among these mutations, changed to His at12, Gln to His at13,respectively.(5) Single nucleotide polymorphism of5’flanking region of INHA gene and of PGR gene were detected. In the two sheep population, no polymorphism was found.(6) Established the best PCR-SSCP methods in the detection of sheep FSHβ gene mutations, under the condition of no report about the polymorphism FSHβ gene in Xinjiang Duolang sheep, single nucleotide polymorphism of exon2of FSHβ gene were detected by PCR-SSCP; AA and AB genotypes were found in GS and KS, there was no significant difference (P>0.05); three genotypes of this gene were found in Duolang sheep; the genotype frequency was:0.57(AA),0.4(AB) and0.03(BB) respectively, and the gene frequency of B and A allele were:0.77and0.23. the average litter size of AA genotype and AB genotype was1.11and1.02lower than those with genotype AB genotype (P<0.05); The sequencing results revealed that six mutations were found, respectively; in FSHβ exon2, C'A, A'*, C'A, A'T, C'A and A'C, at No.102,104,106,107,110and149bp were found, respectively,106C'A,107A'T,110C'A and149A'C, among these mutations, coused His changed to Ile at29, His changed to Ile at29, Ala changed to Glu at30and His changed to Glu at43in exon2FSHβ, respectively.(7) No polymorphism was detected in the amplifide region of PTGS2gene in the two sheep breed (populations). |
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