Identification Of Wheat-Thinopyrum Alien Substitution Lines Using Molecular Cytogenetics

Wild relatives of wheat in Triticeae have contained a great number of such valuable genes as resistant genes and so on, the introduction of aline genetic gemplasm from related species is an important method for improving genetic base of wheat. So the study of the new wheat line CH-9916 has important function to wheat breeding. The new wheat line CH-9916 was identified with cytological marker (Giemsa-C banding, Genome in situ hybridization) and investigation of disease resistance. Results were shown as following:1. The karyotype of materials tested was primarily analyzed by the method of Feulgen counting.The results indicated that genetic composition of all materials tested was stable. The chromosome number of CH-9916 is 2n=42 and its average configuration was 21 II.2.Using the improved Giemsa-C banding technique, there have some imperceptibility difference between CH-9916 and normal wheat, two chromosomes keep accord with Thinopyrum Intermedium character banding in telomeral, others chromosomes keep accord with Sping.3. GISH analysis showed Triticale have 2 items chromosomes presented yellow and others chromosomes presented chrysoidine, because yellow chromosomes combined the Thinopyrum intermedium DNA, others chromosomes dye by PI.4. Analysis of resistance gene: CH-9916 is the hybrid progenies of Octoploid tritielytrigia TAI-7045 X common wheat "Jinmai33" . Octoploid tritielytrigia type TAI-7045 showed immunity to yellow rust and middle resistance powdery mildew. CH-9916 is immune to yellow rust in seedling and adult stages, powdery mildew in seedling and adult stages, and their powdery mildew middle resistance. These suggested that the high powdery mildew-resistance in Octoploid tritielytrigia TAI-7045 had probably been transferred into common wheat.