Studies On The Preparation Of Doxycycline Liposome And Its Pharmacodynamics

In this paper the liposome Doxycycline was made by film disperse-ultrasonic method, by which the encapsulation rate reached to 79.8%, the recollection rate 92% and the liposome have the common size, and have a good stability.Liposome Doxycycline made in this study was tested to determine its inhibitory ability against Escherichia coli C₄₄₁₀₃, Streptococcus O₁₀₂₆, Pasteur bacillus C44-1 and Staphylococcus aureus C₂₆₁₁₂, the result showed the diameter of the inhibition circle were 18.8-22.6 mm, bigger than that of Doxycycline which were 15.9—19.6 mm; The in vitro minimum bacteria inhibition concentration of Liposome Doxycycline was 0.125—0.625μg/ml, smaller than that of Doxycycline which were 0.625—2.5μg/ml; The minimum bacteria killing concentration of Liposome Doxycycline was 1.25—2.5μg/ml smaller than that of Doxycycline which was 2.5--10μg/ml. The result indicated that the in vitro bacteria inhibition ability of liposome Doxycycline was evidently superior to Doxycycline.To study the clinic cure efficacy of Liposome Doxycycline injection, Liposome Doxycycline injection and traditional Doxycycline injection was compared to treat swine enzootic pneumonia and Yellow and white scour of piglets,the results showed the Liposome Doxycycline exhibit an obviously higher cure rate than traditional Doxycycline injection did. Cure rate of swine enzootic pneumonia use high dosage of Liposome Doxycycline was 73.8%,and the effective rate was 95.2%, cure rate of Yellow and white scour of piglets was 87.8 %, effective rate was 95.6%.Further Liposome Doxycycline have the advantages of low use dosage, long-lasting drug effect and low drug residue.