Study On Induction Variation Breeding By Tissue Culture Seedling Chinese Jujube

Combine induction variation and tissue culture, used the tissue culture as material.Hoped to study the induction variation in Chine jujube and screens the polyploid variation department. Establishes the theory and the practice foundation for the jujube tree tissue culture breeding. The major results are as following:1,Cultivates the test tube jujube seedling take the group as the material, take colcthcine the density and the response time as the experimental factor, uses two factors randomizeds block experiment to design, the research fall narcissus cultivates the seedling to thejujube tree group induction variation the effect。The result indicated that, Each processing has the non- diploidproduction, explained the research fall narcissus cultivates the testtube jujube seedling to the group to have a better induction variation function;The experiment screened various varieties group to cultivate theprocessing combination which the jujube seedling comparatively wassuitable: Among them, the dog head jujube for colcthcine 10mg/l,processes 45 day or 50mg/l processes 30 day, induction variation rate is 33.3%; Thesteed jujube for colcthcine 30mg/l, processes 30 day, induction variation rate is40.0%; The wooden jujube for colcthcine 30mg/l, processes 45 day, induction variation rate may reach 46.7%.2,Take tetraploid variation which induction variation produces is and diploid is thegroup cultivates the seedling (comparison) as a material, observesmethod distinction two the and so on the law, leaf area law, blowholelaw with the shape difference。The result indicated that, Tetraploid variation is diploid generallydisplays the leaf blade compared to it to change in a big way, changesthick, changes the circle, the leaf discoloration deeply, the leafsurface shrinkage is rough。The diploid test tube jujube seedling's blowhole density are obviouslymany to a tetraploid variation department, among, the Goutouzao diploid is its induction variation tetraploid 2.05 time, the Junzao diploidis its induction variation tetraploid 1.95 time, the Muzao diploid is its induction variation tetraploid 1.92 time;The polyploid leaf area obviously is bigger than diploid, Goutouzao tetraploid variation leaf area for its diploid 1.78 times,Junzao tetraploid variation leaf area for its diploid 1.33 times,Muzao tetraploid variation leaf area for its diploid 2.59 times ; Tetraploid variation is the blowhole guard-cell chloroplast quantityobvious are many to diploid, among, Goutouzao tetraploidvariation blowhole guard-cell chloroplast quantity for its diploid1.55 times, Junzao tetraploid variation blowhole guard-cellchloroplast quantity for its diploid 1.90 times, Muzao tetraploid variation blowhole guard-cell chloroplast quantity for itsdiploid 2.23 times。3,Take primary election polyploid variation is and diploid is(comparison) as a material. With the paraffin wax cutting movie-making, examines the observation two after the mirror thedifference. The result indicated that, The tetraploid adult plantgenerally displays the leaf blade accumulation, the stockadeorganization, the sponge organization is developed, the stem pointminute fresh level three cells are bigger than originally diploid。4,Take primary election polyploid variation is and diploid is(comparison) as a material, uses the acidolysis to go to the walllowly to infiltrate the law and the enzymolysis goes to the wall lowlyto infiltrate law research tetraploid variation is and diploid is inthe cytogenetics difference。The result indicated that, Two methods both obtained the clearchromosome picture; From the Goutouzao, Muzao, Junzao the three kind of jujube trees groups cultivate thepolyploid variation department's chromosome number which the seedling induction variation obtains may stably raise are 2n=4x=48, compares with originaldiploid 2n=2x=24, the determination is three kind of tetraploidsvariations department。5,Three kind of tetraploids variations department determined which afterprocess screening is a material, take extraneous source plant hormoneas main processing factor, research establishment tetraploid variationdepartment's block traditional form department. The result indicated that, Suits the Goutouzao tetraploid tocontinue a generation of raise the culture medium is 6-BA0.5 +IBA0.2,the increment coefficient is 1.96; Suits Junzao, Muzao tetraploid continues a generation of raise the culture mediumis 6-BA0.5 +IBA0.1, the increment coefficient respectively is 2.03,2.1; Suits the culture medium which the Goutouzao and the Muzao tetraploid takes root is 6-BA0.3 +IAA0.2, takes root to leadrespectively to reach 100% and 82.2%; Suits the culture medium whichthe Junzao tetraploid takes root is 6-BA1.0 +IBA0.3, taking rootrate reaches 100%...