Preliminary Studies On Tissue Culture And Ems Induction Mutation In Phalaenopsis

In this experiments,we choosed to use the inflorescence stalk, leaves and roots of Phalaenopsis in vitro culture to study the protocorm-like-body(PLB) and buds induction.And then study the EMS induction mutation base on these.The results are shown as below:1 The protocorm-like-body and buds induction rate of segment of flower stem knot of different Phalaenopsis variety is different.Among all varietys, "ys-82"variety's induction rate of protocorm-like-body and buds is 46.5%,which is highest,when it was cultured on在MS＋TDZ 1mg/L.2 The protocorm-like-body and buds induction rate of leaves of"ys-82" variety can reach 75%,which is highest.And it is higher then segment of flower stem knot' induction rate.3 During the segment of flower stem knot induct protocorm-like-body(PLB) and buds process,the effect of MS medium is better than 1/2MS, the effect of TDZ is better than 6-BA,and 10% coco milk can increase the induction rate of protocorm-like-body and buds.4 The protocorm-like-body and buds induction rate of segment of flower stem knot of "ys-82" variety is highest and its browning rate when it was treated with 0.75% EMS 1h. The protocorm-like-body and buds induction rate close to 50% when 0.25% EMS treat flower stem for 3h.5 The morphology variation among the regenerative shoot after EMS treatment is almost appeared biserrate leaves tip.6 The resistance of transitory low temperature of regenerative shoot after EMS treatment is weak than regular.But the resistance of durative low temperature of regenerative shoot after EMS treatment is better than regular.The soluble protein content of regeneration shoot is increased when EMS concentration and treatment time is increased.