The ISSR Analysis And HPLC Finger Print Chromatogram Of Discolor Cinquefoil Herb And Medicinal Plants In Potentilla L

Poterntilla discolor Bge, the whole plant of Rosaceae Thunb., is a kind of traditional Chinese medicine. In current time, pharmacological experiments had proved that it had the function of antibacteria, anti-virus and diuresis, and could enhance body immunity. This thesis reviewed the research status of Poterntilla discolor Bge. The active ingredients,pharmacological action and clinical application were analyzed and summarized. The results indicated that its biological activities were high; its effects were positive and worth being further popularized, but basic research should be conducted to develop new herbal preparations.Recently, the research of Poterntilla discolor Bge have been focused on active ingredients,pharmacological action,planting technique and clinical application, but rarely on molecular level. The thesis studied the germplasm resources of Poterntilla discolor Bge elementarily, and the HPLC fingerprints had been constructed.Poterntilla discolor Bge seeds have dormancy property. It is difficult to break the dormancy during the natural conditions. In order to improve the dormancy-breaking process and the germination rate, this study investigated effect on the seed in dormancy-breaking with different methods such as low temperature and other chemical treatments.12 germplasm resources of Poterntilla discolor Bge were used as materials for analyzing their genome polymorphism by ISSR markers. 12 primers screened out from 50 primers were used for ISSR amplification. A total of 128 bands were generated, of which 104 bands were polymorphic bands (the percentage of polymorphic band, PPB=81.25%). Nei'similarity coefficients and genetic distances were calculated by using POPGENE software and the dendrogram were constructed by using UPGMA. The results indicated that there actually existed much genetic diversity on molecular level among the germplasm resources of Poterntilla discolor Bge, the genuieness of Poterntilla discolor Bge was related with the environments in some way. The relationship between genuieness,genetic and environmental factors of Poterntilla discolor Bge were discussed.HPLC fingerprints and a sensitive and specific method is an enssential way to study the herb fingerprint. The gradient elution mode was applied in chromatographic separation. Atlantis C18(250mm×4.6mm,5μm), flow rate: 1.0 mL/ min , detecting wavelength: 368 nm and the column temperature: 25℃.The samples collected from different areas were determined. The samples quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint and this method had a good repeatability and reproducibility. The method can be used to identify and evaluate the quality of Poterntilla discolor Bge conveniently.