Preliminary Study Of Effects On Chicken Intestinal Microflora Community Diversity And Granules Of Concentrated Se By Photosynthetic Bacteria

Photosynthetic bacteria(PSB)is a species of bacteria which can utilize organics as oxygen donator and carbon source to progress photosynthesis without releasing oxygen in condition of oxygen-phobe and illumination. The intestinal microbial population is a group of microorganism which exist in organism's intestinal ,have important physiologic function and don't display dysfunction or pathopoiesis manifestation tract, when organism is healthy. There are some changes in organism's intestinal flora ,when PSB enters into intestinal tract in the shape of viable organism.Selenium (Se) is a very considerable nutritious trace element.Our country is a nation whose soil is generally deficiency of Se, so supplementing Se is a quite momentous for human and animal.But inorganic Se is hard to absorb and has great toxicity, we use PSB to convert inorganic Se into organo-Se which is easy to absorb and has non- toxicity.Thus PSB has dual functions. So, this test study on the cultural condition of converting and concentrating Se by PSB.1 effects on Chicken' intestinal microflora community diversity by Photosy nthetic bacteriaObjective: To comprehend the effects on Chicken' intestinal microflora community diversity by Photosynthetic bacteria and the rule of Chicken' intestinal microflora community diversity change. Methods:Control group select chicken whose ages are 0,3,10,20,30,40,50 to be sample,while PSB group select chicken whose ages are 10,20,40 (stop feeding PSB after 40 ages),50 to be sample, use the method of PCR-DGGE and clone sequence to analysis the diversity change of intestinal microflora community. Result: In section of ileum, diversity index change tendency of control group is that: diversity index from low gradually to advance before 30 ages, then begin to descend when it achieve crest value at 30ages, while it come back after 40 ages. In section of appendix ,diversity index change tendency of control group is that: iversity index from low gradually to advance before 10 ages,it is a platform time between 10 and 30 ages, then begin to descend after 30 ages. In section of rectum,diversity index change tendency of control group is that: diversity index from low gradually to advance before 20 ages, then begin to descend when it achieve crest value at 20 ages, while it come back after30 ages. In section of ileum, diversity index change tendency of PSB group is that: diversity index is the highest one at 10 ages, then it is unremitting descent between 10 and 40 ages,and it is coming back when stop feeding PSB. In section of appendix,diversity index change tendency of PSB group is that: diversity index is slowly advance between 10 and 20 ages, then begin to descend when it achieve crest value at 20 ages. In section of rectum,diversity index change tendency of PSB group is that: diversity index is the highest one at 10 ages, then it is unremitting descent between 10 and 40 ages,and it is coming back when stop feeding PSB.PSB group compare with control group, the descent tendency of diversity index is much better intensity,and all have the tendency to lower than control group in diversity index.Obtained sequences of 10 ages which have autoploidy with 16S rRNA gene of lactobacillus,enterococcus,pseudomonad and Bacillus coli. Obtained three groups of sequences which have autoploidy with 16S rRNA gene of uncultured bacteria. Compared with control group ,PSB group have a band whose sequence has autoploidy with 16S rRNA gene of Bacillus coli and Uncultured proteobacterium.Conclusion: Chicken normal intestinal microflora community diversity is a dynamic change process. PSB is profit at decurtating the time which diversity achieves crest value.But feed PSB for a long time (exceed 10-20days) will degrade intestinal microflora community diversity,so PSB is not fit to continuously feed.At 10 ages of chicken, normal intestinal microflora community in section of ileum is basic established,and there are culturable bacteria and uncultured bacteria in it.2. Research about the cultural conditions of concentrating Se by Photosynthetic bacteriaObjective:Screening of the culture condition of Photosynthetic bacteria with higher transformation efficiency in Se-enriching. Methods: determine Se content by hydride generation-atomic fluorescence spectrophotometry(HG—AFS). Addition of sodium selenite, inoculum size of bacteria and culture time are selected as experiment factors.Select three different level from haplo-factor experiment to contrive orthogonal experiment. Results:Orthogonal Test indicated that culture time and content of sodium selenite were impportant factors that effect the ability of Se-enriched. But the F numerus of these three factors is not significant (P>0.05) .Conclusion:The best enriched-Se Conditions were: 100μg/mL sodium selenite addition ,a 5 days culture,and a inoculum size of 15%.3. Prepared study of granules of concentrated Se by Photosynthetic bacteria Objective:Make PSB of concentrated Se into granule,and comprehend its stability and result of complementing Se .Methods: Wet granulation,detected its quality by vocation standardization,and its viable count in various drying temperature (40℃,50℃,60℃) by plate dilution method. Results:Mass detection all achieved standardization; viable count is decreasing follow with the accrescencing set time and drying temperature,but the decreased amplitude is very small, Se content in blood of concentrated Se group significantly higher than control group' s (P < 0.05) , the Se content in blood of concentrated Se group is unremitting increase,and tend to be stabilization after four days. Conclusion: Optimal drying temperature is 40℃, better stability and conspicuous result in complementing Se .