| The key of realizing the Chinese medicine modernization was to ensure the traditional Chinese medicine had stable chemical composition. At present, because of Salvia miltiorrhiza's widely cultivation and large consumption, more and more attention has been paid to their effective constituents. The experiments were conducted to investigate the effects of organic fertilizers on their plant characters and contents of three kinds of Tanshinone of Salvia miltiorrhiza and to aim at improving their qualities as well.Different fertilizing treatments on sand culture experiment were designed. And Salvia miltiorrhiza and media were sampled on the 80, 110, 140, 170, 200 day after the plants were transplanted. Biology characters, contents of three kinds of Tanshinone and nutrient of root of Salvia miltiorrhiza, contents of available N, available P, available K, available Cu, available Zn, and compounds of phenolic acid in media were determined. The experiment was aimed to study the effect of decomposed substance from manure on growth and accumulation of Tanshinoneâ…¡A, Tanshinoneâ… , and Cryptotanshinone in Salvia Miltiorrhiza's root during its growth period. At the same time, effects of extrinsic hormone and humic acid on growth and accumulation of Tanshinoneâ…¡A, Tanshinoneâ… , and Cryptotanshinone in Salvia Miltiorrhiza's root were studied. Roots of Salvia Miltiorrhiza which were sampled in November, were evaluated by biology characters and contents of three kinds of Tanshinone. This part of experiment was to enrich the study of effects of organic fertilizers on growth and accumulation of Tanshinoneâ…¡A, Tanshinoneâ… , and Cryptotanshinone in Salvia Miltiorrhiza's root The main conclusions are as follows:1. Sand culture experiments showed that organic fertilizers had significant effects on biomass and contents of three kinds of Tanshinone of Salvia miltirrhiza. The effect of organic fertilizer was better than chemical fertilizer on biomass, contents of nutrient and Tashinone. Pig manure was better than moo-cow manure and rape cake on biomass and contents of K, Cu, Zn, Tanshinoneâ…¡A, Tanshinoneâ… , and Cryptotanshinone of Salvia Miltiorrhiza, while cow manure was better on contents of N, and rape cake was better on contents of P.Because the rules of available N, available P, available K, available Cu, available Zn, and compounds of phenolic acid's releasing in media during the decomposing process of different maures were different, the effects of different maures on biomass and content of three kinds of Tanshinone in Salvia miltirrhiza's root were also various. Contents of available N, available Cu and available Zn in media and contents of Tanshinone in Salvia miltirrhiza's root had positive relativity at 0.05 level, while the increasing of contents ofÏ-counmaric acid and ferulic acid in media went against to increasing of biomass and accumulation of Tanshiones in root of Salvia miltiorrhiza.The results of relativity and principal factor analysis demonstrated that biomass and contents of three kinds of Tanshinone in Salvia miltirrhiza's root belong to different principal factors during its growth. And it need a lot of mineral elements during its growing prophase, while it comes to blooming period, the demand to nutrient was small, and microelements made great acting.2. The extrinsic hormone experiment indicated that, treatments of IAA and GA3 under proper concentrate could increase the biomass and contents of three kinds of Tanshinone in root of Salvia Miltiorrhiza. And effects of treatments of GA3 matched with IAA under proper concentrate on biomass and contents of three kinds of Tanshinone in Salvia Miltiorrhiza's root showed added action. In this experiment, treatments of GA3 at concentration of 150 and 300 mg·L-1 and GA3 at concentration of 150 mg·L-1 matched with IAA at concentration of 2 mg·L-1 made against accumulation of three kinds of Tanshinone. In contrast, other treatments increased the quantity of three kinds of Tanshinone, especially treatments of GA3 at concentration of 50 mg·L-1 matched with IAA at 0.5 mg·L-1 who showed largest promoting action on the growth of Salvia Miltiorrhiza. This treatment significantly increased contents of three kinds of Tanshinone in Salvia Miltiorrhiza's root. The effect of treatments of GA3 at concentration of 50 mg·L-1 was in the next place. Along with the increasing of concentrate of IAA, the total quantity of three kinds of Tanshinone increased.3. The extrinsic humic acid experiment showed that, different origins and concentration of humic acid had different effects on growth and accumulation of Tanshinoneâ…¡A, Tanshinoneâ… , and Cryptotanshinone of Salvia Miltiorrhiza. Along with increasing of concentration of humic acid, quantity of Cryptotanshinone, Tanshinoneâ… , Tanshinoneâ…¡A and total quantity of three kinds of Tanshione of Salvia Miltiorrhiza in treatments of humic acid (extracted from Wu Zhatu) reduced, while Cryptotanshinone, Tanshinoneâ… , Tanshinoneâ…¡A and total quantity of three kinds of Tanshione of Salvia Miltiorrhiza in treatments of humic acid (extracted from Ma Gantu) increased. Generally speaking, in this expeirment, treatments of humic acid (extracted from Wu Zhatu )at concentration of 40 mg·L-1 and 80 mg·L-1 and treatments of all humic acid (extracted from Ma Gantu) had better hastening action, especially treatments of humic acid (extracted from Ma Gantu) at concentration of 160 mg·L-1 and humic acid( extract from Wu Zhatu )at concentration of 40 mg·L-1, whose largely promoting action on accumulation of Tanshinone of Salvia Miltiorrhiza's root was more obvious than others. The contents of Cryptotanshinone was the most in treatment of humic acid (extracted from Ma Gantu )at concentrate of 80 mg·L-1. Meanwhile, treatments of humic acid (extracted from Ma Gantu) at concentration of 160 mg·L-1 were most beneficial to accumulation of Tanshinoneâ… and Tanshinoneâ…¡A.
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