Effects Of Fertilization On Effective Chemical Constituents Of Cultivated Salvia Miltiorrhiza Bunge. And Chrysanthemum Morifolium Ramat.

With the more and more quantities of Salvia miltiorrhiza Bunge. and Chrysanthemum morifolium Ramat. being widely cultivated, their effective constituents have being more and more focused on. The experiments were conducted to investigate the effects of fertilization on their effective chemical constituents and to aim at improving their qualities.Salvia miltiorrhiza's effective constituents are inclusive of liposoluble constituents which include tanshinone II a, cryptotanshinone and tanshinone I and water-soluble constituents which include salvianic acid A and protocatechuic aldehyde. These contents were measured by HPLC. Two levels of nutrients (I and II )were supposed in Salvia miltiorrhiza Bunge's pot experiments. N, P and K's amount in each pot is 1.25-0.99-1.46g at nutrient level I , and 2.5-1.98-2.92g at nutrient level II. Organic manure used in the experiments were pig manure, rape cake and organic manure with bacteria. The results could be concluded as follows:Salvia miltiorrhiza's pot experiments showed that contents of tanshinone II a and cryptotanshinone of Salvia miltiorrhiza in pig manure pots and rape cake pots at nutrient level I are higher than those in organic manure with bacteria pots. The contents of salvianic acid A and protocatechuic aldehyde of Salvia miltiorrhiza in pig manure pots and rape cake pots at nutrient level I are also higher than those in organic manure with bacteria pots, however, at nutrient level II the contents of salvianic acid A and protocatechuic aldehyde in organic manure with bacteria pots are higher than those in pig manure pots and rape cake pots.The contents of tanshinone II a and cryptotanshinone of Salvia miltiorrhiza in orgaic manure pots are higher than those in combined application of organic manure and inorganic fertilizer pots. At nutrient level I , the contents of salvianic acid A and protocatechuic aldehyde of Salvia miltiorrhiza in orgaic manure pots are higher than those in combinedapplication of organic manure and inorganic fertilizer pots.The contents of tanshinone IIA and cryptotanshinone of Salvia miltiorrhiza in pig manure pot at nutrient I are the highest among all the pots. It proved that pig manure is better for Salvia miltiorrhiza's effective constituents than rape manure and organic manure with bacteria. This result is consistent with what field experiments proved.Correlation between contents of tanshinone II a and cryptotanshinone is significant at the 0.01 level, correlation between contents of tanshinone II a and tanshinone I is significant at the 0.05 level. Correlation between contents of salvianic acid A and protocatechuic aldehyde is significant at the 0.01 level.Amount of microelements in the experiment improved the contents of tanshinone II a and salvianic acid A of Salvia miltiorrhiza.Dendranthema morifolium's effective constituents includes total flavonoid, which were determined by spectrophotometry. The pot experiments were conducted to investigate the effects oforganic manure on Chrysanthemum morifolium's total flavonoid contents. The results could be concluded as follows:Total flavonoid contents of Chrysanthemum morifolium in pig manure pot are the highest among all the pots, total flavonoid contents in CK and inorganic fertilizer is the lowest.The stems and leaves of Chrysanthemum morifolium also contain flavonoid. The total flavonoid content of flower, stem and leaf was 5.66%, 3.10% and 1.44%, respectively.The total flavonoid content of bud, flower in blossoming 50%, flower in blossoming 70% and flower in full bloom was 6.12%, 5.27%, 5.95% and 5.66%. The total flavonoid content of different blossoming degrees: bud>flower in blossoming 70%>flower in full bloom>flower in blossoming 50%.