Classification And Evaluation Of Three Composite Wild Vegetables Resources In Nanjing

There was a tradition of eating wild vegetables in Nanjing. Because of having beeneared widly, Artemisia selengensis Turca., Chrysanthemum nankingense (Hand.-Mazz.)X.D.Cui, Kalimeris indica (L.) Sch.-Bip., Capsella bursa-pastoris (L.)Mddic., Medicagohispida Gaertn., Portulaca oleracea L., Lycium Chinese Mill., and Toona sinensis (AJuss.)Roem. have been called the eight wild vegetables in Nanjing.In this study, we mainly researched Artemisia selengensis Turca., Chrysanthemumnankingense (Hand.-Mazz.)X.D.Cui and Kalimeris indica (L.) Sch.-Bip. These three kindsof vegetables are eated and cultivated most wildly.All of the three kinds which belong toComposite and have special flavour are cultured and eaten widly in Nanjing.Firstly wecollected materials with different morphology in Nanjing area, and classified them initiallyby morphlogical charactors and isoenzyme techenology.Then the classified results wereidentified by RAPD.Genetic diversity and cluster analysis were carried by biologicalsoftware to discuss the intraspecific variation and genetic relationship.Different types of thematerirals were evaluated by investgating field agrinomic charactors and measuringnutrient content to accumulate information for further use.The main results of the research are as follows:We have collected four different Artemisia selengensis Turca., all of which werefanaahold cultivars in Nanjing.The results of morphological classification, isoenzymeclassification and RAPD identification were coincident.It made sure that these fourmaterial were different types.The result of genetic diversity analysis showed that theintraspecies variation of Artemisia selengensis Turca. was not much higher. According to theinformation of isoenzyme bands, the results of field investigation and nitrition content, wethought Fuqiu green and Dongchun green would be higher valuable for resolving theproduction problems.It made sure that the five Chrysanthemum nankingense (Hand.-Mazz.) X.D. Cui,materials collected belonged to five different types by morphlogical characters comparation, isoenzyme analysis and RAPD.The result of genetic diversity analysis showed that therewould be higher intraspecies variation in Chrysanthemum nankingense (Hand.-Mazz.) X.D.Cui.The agrinomic characters and nitrition content were assessed.It found that thedifference of agrinomic characters was not distinct, but the content difference of somenitrifion was obvious.So the nitrition content could be an important index to select propervarity.The materials of Kalimeris indica(L.)Sch.-Bip. have been divided into eight types byRAPD. It's according with the identification result of morphological identification andisoenzyme analysis.The result of genetic diversity analysis showed that there would bemore intraspecies variation in Kalimeris indica(L.)Sch.-Bip. The agrinomic characters andnitrition content were assessed.There existed a little difference among the eight materials inthe ability of drought-resistant, multiplication, early florescence, et al., but the differenceof nutrition content were much significant.So we can select appropriate material for use ofproduction and research according to the need.In order to improve propagation ability, we investigated the tissue culture and rapidpropagation of shoot tips of kalimeris indica (L) Sch.-Bip in vitro.The results showed thatbest medium for inducing and multiplication of shoots was MS medium containing 0.5mg·L^-1 6-BA, 3％sucrose and 0.78％agar.The best medium for inducing roots was 1/2MSmedium containing 2.0 mg·L^-1 IBA, 3％sucrose and 0.78％agar with PH 5.8.Themediums which we have screened out would provide spadework for rapid propagation ofkalimeris indica (L) Sch.-Bip.